Recombinant Human DMBT1 His-tag Protein, CF

When Recombinant Human Siglec-8 Fc Chimera Protein (9045-SL) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Human DMBT1 His-tag (Catalog # 11032-DB) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL

2 μg/lane of Recombinant Human DMBT1 His-tag Protein (Catalog # 11032-DB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 225-285 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DMBT1 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-8 Fc Chimera Protein (Catalog # 9045-SL) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Human DMBT1 His-tag (Catalog # 11032-DB) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human DMBT1 protein
  Thr20-Arg1662 (T31A, T42P, L54S, S1629L), with a C-terminal 6-His tag
• Accession #: AAI53300.1
• N-terminal Sequence: Thr20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 225-285 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DMBT1 His-tag Protein, CF

• CRP-ductin
• deleted in malignant brain tumors 1 protein
• deleted in malignant brain tumors 1
• DMBT1
• Ebnerin
• FLJ61058
• Glycoprotein 340
• GP340
• gp-340
• GP340muclin
• Hensin
• MGC164738
• Muclin
• SAG
• Salivary agglutinin
• Surfactant pulmonary-associated D-binding protein
• Vomeroglandin

Background

DMBT1 (Deleted in Malignant Brain Tumor 1) also known as Glycoprotein 340 and salivary agglutinin is a secreted glycoprotein with mass of 340 kDa. It belongs to group B of the SRCR (scavenger cysteine-rich) superfamily with each SRCR domain containing 8 cysteines (1). Primary sequence of DMBT1 is composed of 8-13 SRCR domains (due to alternative splicing), a CUB domain, another SRCR domain, a second CUB domain and ended with a ZP (zona pellucida) domain (2-3). DMBT1 is heavily glycosylated where N-linked and O-linked glycosylation account for almost 25% of the mass (3-4). It is mainly expressed in epithelial cells and mucosal tissues of respiratory and gastrointestinal tracts and urogenital organs. It is also found in human body fluids (saliva, tear, broncho-alveolar lavage, and pancreatic juice) (5-6). Extent of glycosylation varies among different tissues or individuals (7-8). Orthologues of DMBT1 have been found in monkey, mouse, rat, rabbit, bovine, and porcine. Human DMBT1 shares 49% and 35% amino acid homology with mouse and rat homolog. DMBT1 plays an important role in innate immune response. It is an agglutinating agent for several bacteria strains through a specific region in the SRCR domain in a calcium dependent way (9-10). It can also bind to viruses such as influenza A virus and human immunodeficiency virus type I and inhibit their activity. Binding to viruses is mediated by the sialic acid glycan of DMBT-1, independent of calcium. In addition to binding pathogens, DMBT-1 also binds a broad spectrum of host proteins including SP-d, SP-A, secretory IgA, refoil factors (TFFs), MUC5B, complement factor C1q, lactoferrin and DNA (11). Recent studies identify DMBT1 as a high affinity ligand of Siglec-8 in human airways (12). Inactivation of DMBT1 affects stem cell differentiation and results in tumor formation (13).

1. Resnick, D. et al. (1994) Trends Biochem Sci. 19:5.
2. Holmskov, U. et al. (1999) Proc Natl Acad Sci USA. 96:10794.
3. Mollenhauer, J. et al. (1997) Nat Genet. 17:32.
4. Prakobphol, A. et al. (2000) J Biol Chem. 275:39860.
5. Mollenhauer, J. et al. (2000) Cancer Res. 60:1704.
6. Stoddard, E. et al. (2007) J Immunol. 179:3126.
7. Schulz, B. L. et al. (2002) Biochem J. 366:511.
8. Eriksson, C. et al. (2007) Glycoconj J. 24:131.
9. Bikker, F.J. et al. (2004) J Biol Chem. 279:47699.
10. Madsen, J. et al. (2003) Eur J Immunol. 33:2327.
11. Madsen, J. et al. (2010) Innate Immun. 16:160.
12. Gonzalez-Gil, A. et al. (2021) J Allergy Clin Immunol. 147:1442.
13. Ligtenberg, A. J. et al. (2007) Biol Chem. 388:1275.

Bioinformatics

• Uniprot:
  • AAI53300.1()