Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

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Product Details

Summary
Reactivity Hu, Cm, RMSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived CD28 protein
Human/Cynomolgus Monkey/Rhesus Macaque CD28
(Asn19-Pro152)
Accession # P10747
DMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Asn19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CD28
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
42.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-70 kDa, reducing conditions
Publications
Read Publications using
342-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44

Background

CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the
up-regulation of bcl-XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.

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342-CD
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Applications: Bioactivity

Publications for CD28 (342-CD)(6)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 4 applications: Bioassay, ELISA Standard, ELISA capture, Flow.


Filter By Application
Bioassay
(3)
ELISA Standard
(1)
ELISA capture
(1)
Flow
(1)
All Applications
Filter By Species
Human
(3)
N/A
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using 342-CD Applications Species
UA Ramagopal, W Liu, SC Garrett-Th, JB Bonanno, Q Yan, M Srinivasan, SC Wong, A Bell, S Mankikar, VS Rangan, S Deshpande, AJ Korman, SC Almo Structural basis for cancer immunotherapy by the first-in-class checkpoint inhibitor ipilimumab Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28484017] (Bioassay, N/A) Bioassay N/A
Haanstra K, Dijkman K, Bashir N, Bauer J, Mary C, Poirier N, Baker P, Scobie L, 't Hart B, Vanhove B Selective blockade of CD28-mediated T cell costimulation protects rhesus monkeys against acute fatal experimental autoimmune encephalomyelitis. J Immunol, 2015;194(4):1454-66. 2015 [PMID: 25589073] (Bioassay) Bioassay
Kowalczyk A, D'Souza C, Zhang L Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol, 2014;44(4):1143-55. 2014 [PMID: 24338929] (Bioassay, Human) Bioassay Human
Sakthivel P, Shively V, Kakoulidou M The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 2007;261(4):399-407. 2007 [PMID: 17391115] (ELISA Standard, N/A) ELISA Standard N/A
Neuber K, Mahnss B, Hubner C, Gergely H, Weichenthal M Autoantibodies against CD28 are associated with atopic diseases. Clin. Exp. Immunol., 2006;146(2):262-9. 2006 [PMID: 17034578] (ELISA capture, Human) ELISA capture Human
Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells. J. Biol. Chem., 2002;277(41):38660-8. 2002 [PMID: 12167647] (Flow, Human) Flow Human

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Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD28
Entrez
Uniprot