Recombinant Human CXCL11/I-TAC Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human CXCL11/I-TAC Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED₅₀ for this effect is 1‑5 ng/mL.
• Source: E. coli-derived human CXCL11/I-TAC protein
  Phe22-Phe94
• Accession #: O14625
• N-terminal Sequence: Phe22
• Protein/Peptide Type: Recombinant Proteins
• Gene: CXCL11
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 8.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL11/I-TAC Protein

• beta-R1
• b-R1
• chemokine (C-X-C motif) ligand 11
• CXCL11
• H174
• H174IP9
• Interferon gamma-inducible protein 9
• Interferon-inducible T-cell alpha chemoattractant
• IP-9member 11
• ITAC
• I-TAC
• I-TACMGC102770
• SCYB9B
• small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• Small-inducible cytokine B11

Background

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

1. Cole, K. et al. (1998) J. Exp. Med. 187:2009.
2. Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
3. Lou, Y. et al. (1998) J. Neurovirol. 4:575.

Publications for CXCL11/I-TAC (672-IT)(17)

Publications using 672-IT

• Kim, JY;Cho, DW;Choi, JY;Jeong, S;Kang, M;Kim, WJ;Hong, IS;Song, H;Chang, H;Yang, SR;Lee, SJ;Park, M;Hong, SH; CXCL11 reprograms M2-biased macrophage polarization to alleviate pulmonary fibrosis in mice Cell & bioscience 2024-11-15 [PMID: 39548525] (In vivo assay, Mouse)
• ST Kim, Y Chu, M Misoi, ME Suarez-Alm, JH Tayar, H Lu, M Buni, J Kramer, E Rodriguez, Z Hussain, SS Neelapu, J Wang, AY Shah, NM Tannir, MT Campbell, DL Gibbons, T Cascone, C Lu, GR Blumensche, M Altan, B Lim, V Valero, ME Loghin, J Tu, SN Westin, A Naing, G Garcia-Man, N Abdel-Waha, HA Tawbi, P Hwu, ICG Oliva, MA Davies, SP Patel, J Zou, A Futreal, A Diab, L Wang, R Nurieva Distinct molecular and immune hallmarks of inflammatory arthritis induced by immune checkpoint inhibitors for cancer therapy Nature Communications, 2022-04-12;13(1):1970. 2022-04-12 [PMID: 35413951] (Bioassay, Human)
• N Lotfi, GX Zhang, N Esmaeil, A Rostami Evaluation of the effect of GM-CSF blocking on the phenotype and function of human monocytes Sci Rep, 2020-01-31;10(1):1567. 2020-01-31 [PMID: 32005854] (Cell Culture, Human)
• F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019-10-24;20(21):. 2019-10-24 [PMID: 31653015] (Bioassay, Human)
• Hsiao J, Ng B, Smits M, Wang J, Jasavala R, Martinez H, Lee J, Alston J, Misonou H, Trimmer J, Wright M Androgen receptor and chemokine receptors 4 and 7 form a signaling axis to regulate CXCL12-dependent cellular motility. BMC Cancer, 2015-03-31;15(0):204. 2015-03-31 [PMID: 25884570] (Bioassay, Human)
• Liu , Che, Pham , Kien, Luo , Defang, Reynolds , Brent A, Hothi , Parvinde, Foltz , Gregory, Harrison , Jeffrey Expression and functional heterogeneity of chemokine receptors CXCR4 and CXCR7 in primary patient-derived glioblastoma cells. PLoS ONE, 2013-03-21;8(3):e59750. 2013-03-21 [PMID: 23555768] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Berahovich RD, Zabel BA, Penfold ME, Lewen S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ CXCR7 protein is not expressed on human or mouse leukocytes. J. Immunol., 2010-10-01;185(9):5130-9. 2010-10-01 [PMID: 20889540] (Binding Assay, Human)
• Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B. Immunology, 2009-12-02;129(4):536-46. 2009-12-02 [PMID: 20002784] (Bioassay, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Bioassay, Human)
• Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation. Blood, 2008-07-21;112(7):2648-56. 2008-07-21 [PMID: 18645041] (Bioassay, Hamster)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007-09-15;179(6):4272-82. 2007-09-15 [PMID: 17785868] (Bioassay, Human)
• Proost P, Mortier A, Loos T, Vandercappellen J, Gouwy M, Ronsse I, Schutyser E, Put W, Parmentier M, Struyf S, Van Damme J Proteolytic processing of CXCL11 by CD13/aminopeptidase N impairs CXCR3 and CXCR7 binding and signaling and reduces lymphocyte and endothelial cell migration. Blood, 2007-03-15;110(1):37-44. 2007-03-15 [PMID: 17363734] (Bioassay, Human)
• Nguyen AN, Stebbins EG, Henson M, O&amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. Exp. Cell Res., 2006-04-04;312(10):1909-23. 2006-04-04 [PMID: 16600214] (Bioassay, Human)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Bioassay, Human)
• Liu L, Jarjour NN, Busse WW, Kelly EA Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24. 2004-03-04 [PMID: 15001464] (ELISA (Standard))
• Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding. Eur. J. Pharmacol., 2002-12-05;456(1):1-10. 2002-12-05 [PMID: 12450563] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CXCL11
• Uniprot:
  • O14625()