Recombinant Human CTRP9/C1qTNF9 Protein, CF

When Recombinant Human CTRP9/C1qTNF9 (Catalog # 6537-TN) is coated at 0.5 μg/mL, 100 μL/well, Biotinylated Recombinant Human CL-K1/COLEC11 binds with an ED50 of 0.035-0.21 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CTRP9/C1qTNF9 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human CTRP9/C1qTNF9 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CL-K1/COLEC11 that produces 50% of the optimal binding response is 0.035-0.21 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human CTRP9/C1qTNF9 protein Asn16-Pro333, with an N-terminal 6-His tag
• Accession #: AAH40438
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: C1QTNF9
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CTRP9/C1qTNF9 Protein, CF

• AQL1
• C1q and tumor necrosis factor related protein 9
• C1qTNF9
• C1QTNF9AComplement C1q tumor necrosis factor-related protein 9
• complement C1q tumor necrosis factor-related protein 9A
• CTRP9
• MGC48915

Background

C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Human C1qTNF9 shares 85% amino acid sequence identity with the mouse and rat orthologs. Both the mouse and human C1qTNF9 proteins are expressed in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). They have both also been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet-induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).

1. Wong, G.W. et al. (2009) FASEB J. 23:241.
2. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
3. Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.
4. Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.
5. Su, H. et al. (2013) Basic Res. Cardiol. 108:315.
6. Sun, Y. et al. (2013) Circulation 128:S113.
7. Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305:R522.
8. Hwang, Y.C. et al. (2013) Int. J. Obes. (Lond) [Epub ahead of print].

Publications for CTRP9/C1qTNF9 (6537-TN)(1)

Publication using 6537-TN

• Q Liu, H Zhang, J Lin, R Zhang, S Chen, W Liu, M Sun, W Du, J Hou, B Yu C1q/TNF-related protein 9 inhibits the cholesterol-induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating AMP-dependent kinase J. Cell. Mol. Med., 2017-05-19;0(0):. 2017-05-19 [PMID: 28524645] (Bioassay, Human)

Bioinformatics

• Gene Symbol: C1QTNF9
• Uniprot:
  • AAH40438()