Recombinant Human CDO Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human CDO Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When rhSHH (2 µg/mL) is incubated with serial dilutions of rhCDO in solution, rhCDO binds rhSHH with an apparent K D <30 nM.
• Source: Mouse myeloma cell line, NS0-derived human CDO protein Asp26-Pro943 (Leu669Ile), with a C-terminal 6-His tag
• Accession #: NP_058648
• N-terminal Sequence: Asp26
• Protein/Peptide Type: Recombinant Proteins
• Gene: CDON
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 100.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-140 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CDO Protein, CF

• CDO
• Cdon homolog (mouse)
• CDON
• CDOORCAM
• cell adhesion molecule-related/down-regulated by oncogenes
• MGC111524
• ORCAM
• surface glycoprotein, Ig superfamily member

Background

CDO (CAM-related/down-regulated by oncogenes, also CDON; pronounced “kid-oh”) is a 190 kDa member of the Immunoglubulin (Ig) superfamily, Ig/Fibronectin (FN) type III repeat family of cell surface proteins (1). Human CDO is a type I transmembrane (TM) glycoprotein. It is synthesized as a 1287 amino acid (aa) precursor that contains a 25 aa signal sequence, a 938 aa extracellular domain (ECD), a 21 aa TM segment and a 303 aa cytoplasmic region (1, 2). The ECD contains five C2-type Ig-like domains, followed by three FN type III repeats. The first FN repeat (aa 577 - 673) is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat (aa 826 - 923) binds SHH (3, 4). The intracellular region is believed to signal through various bHLH transcription factors (2). One alternate splice form is reported that shows a deletion of aa 1212 - 1234 in the cytoplasmic tail. The ECD of human CDO is 85% aa identical to mouse CDO ECD. CDO is found on muscle precursor and neural progenitor cells of the embryo (5, 6). It likely promotes muscle differentiation, and contributes to axon guidance and neuronal patterning (2, 7, 8, 9). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (2). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly-defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (7, 8, 10).

1. Kang, J.S. et al. (1997) J. Cell Biol. 138:203. 
2. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355. 
3. Yao, S. et al. (2006) Cell 125:343. 
4. 4.Kang, J-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989. 
5. Kang, J-S. et al. (2002) EMBO J. 21:114. 
6. Zhang, W. et al. (2006) Mol. Cell. Biol. 26:3764.
7. Okada, A. et al. (2006) Nature 444:369.
8. Allen, B.L. et al. (2007) Genes Dev. 21:1244.
9. Kang, J-S. et al. (2004) J. Cell. Biol. 167:493.
10. Tenzen, T. et al. (2006) Dev. Cell 10:647.

Bioinformatics

• Gene Symbol: CDON
• Uniprot:
  • NP_058648()