Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

2 μg/lane of Recombinant Human CD68/SR-D1 Fc Chimera (Catalog # 10301-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 116-130 kDa and 230‑260 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human S100A8 (Catalog # 9876-S8) is immobilized at 10 µg/mL (100 µL/well), it binds to Recombinant Human CD68/SR‑D1 Fc Chimera with an ED₅₀ of 5-30 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human CD68/SR-D1 protein
  

  Human CD68/SR-D1 (Asn22-Ile320) Accession # P34810	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P34810
• N-terminal Sequence: Asn22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 116-130 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

• CD_antigen: CD68
• CD68 antigen
• CD68 molecule
• CD68
• DKFZp686M18236
• gp110
• LAMP4
• macrophage antigen CD68
• Macrosialin
• SCARD1
• scavenger receptor class D, member 1
• SRD1
• SR-D1

Background

CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family (1). Human CD68 contains 354 amino acids (aa), with a 21 aa signal sequence and a 298 aa extracellular domain (ECD), a 25 aa transmembrane domain, and a 10 aa cytoplasmic domain. The ECD of CD68 has 9 glycosylation sites and a mucin-like region in aa 23-140. Within the ECD, human CD68 shares a 65% and 67% amino acid sequence identity with that of mouse and rat CD68, respectively. CD68 is expressed on monocytes and tissue macrophages and serves as a scavenger receptor for oxidized LDL (2). In contrast to other members of the LAMP-family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appeared to have a more restricted, cell-type specific pattern of expression (3 ,4). CD68 is also a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (5, 6). CD68 influences the functions of cells through NF-kB/focal adhesion kinase pathway (7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity (8).

1. Holness, C.L., Simmons, D.L. (1993) Blood 81:1607.
2. Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
3. Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453
4. Chistiakov, D. et al. (2016) Lab Invest. 97:13.
5. Bresnihan, B. et al. (2009) J Rheumatol. 36:1800.
6. Touati, M. et al. (2015) 56:332.
7. Wang, H. et al. (2014) Cancer Lett. 345:91.
8. Okada, K. et al. (2016) J. of Leukoc. Bio. 100:1093.

Publications for CD68/SR-D1 (10301-SR)(1)

Publication using 10301-SR

• Li, X;Yan, Y;Wang, Z;Hou, J;Meng, Y;Cui, D;Long, Y;Li, M;Yang, R; The link between osteoporosis and frozen shoulder: exploring the therapeutic effect of TAK715 on reversing fibrosis and protecting against osteoporosis via the p38 MAPK signaling pathway BMC musculoskeletal disorders 2024-11-21 [PMID: 39574076] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P34810()