Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged)


Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) (Catalog #2706-CL) stimulates cell proliferation using enriched B cells in the presence ofIL-4. The ED50 for this effect is 2-12 ng/mL in the presence of 10 μg/mL more
1 μg/lane of Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) Summary

Additional Information
A New rh CD40 Ligand is Now Available! ~10 fold better activity; HEK293 expressed.
Details of Functionality
Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 2‑12 ng/mL in the presence of 10 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) and 20 ng/mL of recombinant human IL-4.
E. coli-derived human CD40 Ligand/TNFSF5 protein
Glu108-Leu261, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
17.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
18 kDa, reducing conditions
Read Publications using
2706-CL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and EDTA with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged)

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5


CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily (1-3). Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region (4, 5). The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms (6-9). The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable (8). CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells (10). The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells (11).  CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (12, 13). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (14, 15). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (16, 17).
  1. Zhang, G. (2004) Curr. Opin. Struct. Biol. 14:154.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Quezada, S.A. et al. (2004) Annu. Rev. Immunol. 22:307.
  4. Graf, D. et al. (1992) Eur. J. Immunol. 22:3191.
  5. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
  6. Khandekar, S.S. et al. (2001) Protein Expr. Purif. 23:301.
  7. Pietravalle, F. et al. (1996) J. Biol. Chem. 271:5965.
  8. Garber, E. et al. (1999) J. Biol. Chem. 274:33545.
  9. Vakkalanka, R.K. et al. (1999) Arthritis Rheum. 42:871.
  10. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  11. Eissner, G. et al. (2004) Cytokine Growth Factor. Rev. 15:353.
  12. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  13. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  14. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  15. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  16. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  17. Hassan, G.S. et al. (2012) Immunobiology 217:521.

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Publications for CD40 Ligand/TNFSF5 (2706-CL)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.

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Showing Publications 1 - 6 of 6.
Publications using 2706-CL Applications Species
W Rénier, A Bourdin, PA Rubbo, M Peries, L Dedieu, S Bendriss, L Kremer, S Canaan, D Terru, S Godreuil, N Nagot, P Van de Per, E Tuaillon B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections PLoS ONE, 2018-04-30;13(4):e0196470. 2018-04-30 [PMID: 29709002] (Bioassay, Human) Bioassay Human
M Schoenhals, M Jourdan, A Bruyer, A Kassambara, B Klein, J Moreaux Hypoxia favors the generation of human plasma cells Cell Cycle, 2017-05-02;0(0):1-14. 2017-05-02 [PMID: 28463531] (Bioassay, Human) Bioassay Human
Posttranscriptional upregulation of p53 by reactive oxygen species in chronic lymphocytic leukemia Cancer Res, 2016-09-07;0(0):. 2016-09-07 [PMID: 27634759] (Bioassay, Human) Bioassay Human
Cavallini C, Lovato O, Bertolaso A, Pacelli L, Zoratti E, Zanolin E, Krampera M, Zamo A, Tecchio C, Cassatella M, Pizzolo G, Scupoli M The TNF-family cytokine TL1A inhibits proliferation of human activated B cells. PLoS ONE, 2013-04-02;8(4):e60136. 2013-04-02 [PMID: 23565196] (Bioassay, Human) Bioassay Human
Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow. PLoS ONE, 2012-05-02;7(5):e35639. 2012-05-02 [PMID: 22567106] (Bioassay, Human) Bioassay Human
Celis E, Celis E Overlapping human leukocyte antigen class I/II binding peptide vaccine for the treatment of patients with stage IV melanoma: evidence of systemic immune dysfunction. Cancer, 2007-07-01;110(1):203-14. 2007-07-01 [PMID: 17541944] (Bioassay, Human) Bioassay Human

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FAQs for CD40 Ligand/TNFSF5 (2706-CL). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at with any questions regarding this program.

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Gene Symbol CD40LG