Recombinant Human CCL21/6Ckine Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CCL21/6Ckine Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.2-0.6 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived human CCL21/6Ckine protein
Ser24-Pro134
Accession #
N-terminal Sequence
Ser24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL21
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
16-17 kDa, reducing conditions
Publications
Read Publications using
366-6C/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL21/6Ckine Protein, CF

  • 6Ckine
  • 6CkineSmall-inducible cytokine A21
  • Beta-chemokine exodus-2
  • CCL21
  • chemokine (C-C motif) ligand 21
  • CKb9
  • exodus-2
  • member 21
  • SCYA21
  • SCYA21MGC34555
  • secondary lymphoid tissue chemokine
  • SLC
  • SLCSecondary lymphoid-tissue chemokine
  • TCA-4

Background

CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a 12 kDa homeostatic chemokine that plays an important role in adaptive immune responses and inflammation (1). Unlike other CC chemokines, human CCL21 has a 37 amino acid (aa) C-terminal extension which mediates its attachment to carbohydrate structures and extracellular matrix components (2, 3). Mature human CCL21 shares 71% and 66% aa sequence identity with mouse and rat CCL21, respectively (4‑6). Both human and mouse CCL21 signal through the chemokine receptor CCR7, while mouse CCL21 additionally can signal through CXCR3 (7). CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) (8‑10). Immobilized CCL21 promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation (8, 9). DC interaction with the anchored chemokine can induce CCL21 cleavage and release of an 8 kDa fragment that lacks the C-terminal extension (10). During chronic inflammation or tissue damage, CCL21 is up‑regulated on local vascular endothelial cells, macrophages, T cells, and neurons (11‑14). In these settings, it promotes fibrosis, inflammatory cytokine production, and neuropathic pain (12‑14). The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients (11, 13). CCL21 has been shown to exert either angiogenic or angiostatic effects (11, 15, 16). These effects, in combination with the ability of CCL21 to attract immune suppressor cells (Treg and MDSC) to a tumor site can have positive or negative effects on tumor progression (16, 17).
  1. Forster, R. et al. (2008) Nat. Rev. Immunol. 8:362.
  2. Yang, B.G. et al. (2007) J. Immunol. 179:4376.
  3. Rey-Gallardo, A. et al. (2010) Glycobiology 20:1139.
  4. Hromas, R. et al. (1997) J. Immunol. 159:2554.
  5. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.
  6. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
  7. Jenh, C. et al. (1999) J. Immunol. 162:3765.
  8. Tal, O. et al. (2011) J. Exp. Med. 208:2141.
  9. Friedman, R.S. et al. (2006) Nat. Immunol. 7:1101.
  10. Schumann, K. et al. (2010) Immunity 32:703.
  11. Pickens, S.R. et al. (2012) Arthritis Rheum. 64:2471.
  12. Sakai, N. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14098.
  13. Damas, J.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:614.
  14. Biber, K. et al. (2011) EMBO J. 30:1864.
  15. Soto, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8205.
  16. Vicari, A.P. et al. (2000) J. Immunol. 165:1992.
  17. Shields, J.D. et al. (2010) Science 328:749.

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366-6C/CF
Species: Hu
Applications: Bioactivity

Publications for CCL21/6Ckine (366-6C/CF)(19)

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(1)
Bioassay
(18)
All Applications
Filter By Species
Human
(18)
Virus
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using 366-6C/CF Applications Species
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (Bioassay, Human) Bioassay Human
JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use J Immunol Res, 2017;2017(0):1975902. 2017 [PMID: 28785596] (Bioassay, Human) Bioassay Human
Alison Swaims-Koh Progesterone Levels Associate with a Novel Population of CCR5+CD38+ CD4 T Cells Resident in the Genital Mucosa with Lymphoid Trafficking Potential J Immunol, 2016;197(1):368-76. 2016 [PMID: 27233960] (Bioassay, Human) Bioassay Human
Yu, Qing, Lou, Xiang-mi, He, Yan Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway. PLoS ONE, 2015;10(3):e0120855. 2015 [PMID: 25768730] (Bioassay, Human) Bioassay Human
Tutunea-Fatan, Elena, Majumder, Mousumi, Xin, Xiping, Lala, Peeyush The role of CCL21/CCR7 chemokine axis in breast cancer-induced lymphangiogenesis. Mol Cancer, 2015;14(1):35. 2015 [PMID: 25744065] (Bioassay, Human) Bioassay Human
Till K, Pettitt A, Slupsky J Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase delta but not SYK or BTK in chronic lymphocytic leukemia cells. J Immunol, 2015;194(5):2439-46. 2015 [PMID: 25632006] (Bioassay, Human) Bioassay Human
Borgman K, van Zanten T, Manzo C, Cabezon R, Cambi A, Benitez-Ribas D, Garcia-Parajo M Priming by chemokines restricts lateral mobility of the adhesion receptor LFA-1 and restores adhesion to ICAM-1 nano-aggregates on human mature dendritic cells. PLoS ONE, 2014;9(6):e99589. 2014 [PMID: 24945611] (Bioassay, Human) Bioassay Human
Xu Y, Zhang M, Ramos C, Durett A, Liu E, Dakhova O, Liu H, Creighton C, Gee A, Heslop H, Rooney C, Savoldo B, Dotti G Closely related T-memory stem cells correlate with in vivo expansion of CAR.CD19-T cells and are preserved by IL-7 and IL-15. Blood, 2014;123(24):3750-9. 2014 [PMID: 24782509] (Bioassay, Human) Bioassay Human
Irino, Tomoyuki, Takeuchi, Hiroya, Matsuda, Sachiko, Saikawa, Yoshiro, Kawakubo, Hirofumi, Wada, Norihito, Takahashi, Tsunehir, Nakamura, Rieko, Fukuda, Kazumasa, Omori, Tai, Kitagawa, Yuko CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma. BMC Cancer, 2014;14(0):291. 2014 [PMID: 24766770] (Bioassay, Human) Bioassay Human
Da Silva D, Movius C, Raff A, Brand H, Skeate J, Wong M, Kast W Suppression of Langerhans cell activation is conserved amongst human papillomavirus alpha and beta genotypes, but not a micro genotype. Virology, 2014;452(0):279-86. 2014 [PMID: 24606705] (Bioassay, Human) Bioassay Human
Show All 19 Publications.

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Bioinformatics

Gene Symbol CCL21
Entrez
Uniprot