Recombinant Human CCL1/I-309 Protein, CF

Images

 
There are currently no images for CCL1/I-309/TCA-3 (272-I/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human CCL1/I-309 Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BW5147 mouse T lymphoma cells. The ED50 for this effect is 3-9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR8. The ED50 for this effect is 1.5-7.5 ng/mL.
Source
E. coli-derived human CCL1/I-309/TCA-3 protein
Lys24-Lys96
Accession #
N-terminal Sequence
Lys24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
272-I/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL1/I-309 Protein, CF

  • C-C motif chemokine 1
  • CCL1
  • chemokine (C-C motif) ligand 1
  • I-309
  • inflammatory cytokine I-309
  • P500
  • SCYA1
  • SCYA1Small-inducible cytokine A1
  • SCYA2
  • SISe
  • small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
  • T lymphocyte-secreted protein I-309
  • TCA3
  • TCA-3

Background

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The sequence of CCL1 contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15 - 16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

  1. Miller, M.D. et al. (1992) Proc. Natl. Acad. Sci. USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.

Customers Who Viewed This Item Also Viewed...

6507-IL/CF
Species: Hu
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-37856
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB1567
Species: Hu
Applications: Flow, CyTOF-reported
272-I/CF
Species: Hu
Applications: Bioactivity

Publications for CCL1/I-309/TCA-3 (272-I/CF)(8)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 2 applications: Bioassay, ELISA Standard.


Filter By Application
Bioassay
(7)
ELISA Standard
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
N/A
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 272-I/CF Applications Species
Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28533380] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011;12(2):167-77. 2011 [PMID: 21217759] (Bioassay, Mouse) Bioassay Mouse
Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM CC chemokine ligand 1 is released into the airways of atopic asthmatics. Eur. Respir. J., 2006;28(1):59-67. 2006 [PMID: 16540498] (Bioassay, Human) Bioassay Human
Ruffini PA, Biragyn A, Coscia M, Harvey LK, Cha SC, Bogen B, Kwak LW Genetic fusions with viral chemokines target delivery of nonimmunogenic antigen to trigger antitumor immunity independent of chemotaxis. J. Leukoc. Biol., 2004;76(1):77-85. 2004 [PMID: 15075363] (Bioassay, Human) Bioassay Human
Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002;168(12):6244-52. 2002 [PMID: 12055238] (Bioassay, Human) Bioassay Human
Cashman J, Clark-Lewis I, Eaves A, Eaves C Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice. Blood, 2002;99(3):792-9. 2002 [PMID: 11806978] (Bioassay, Human) Bioassay Human
Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. J. Leukoc. Biol., 2001;70(4):617-23. 2001 [PMID: 11590199] (ELISA Standard, N/A) ELISA Standard N/A

Reviews for CCL1/I-309/TCA-3 (272-I/CF) (0)

There are no reviews for CCL1/I-309/TCA-3 (272-I/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CCL1/I-309/TCA-3 (272-I/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for CCL1/I-309/TCA-3 (272-I/CF)

Discover related pathways, diseases and genes to CCL1/I-309/TCA-3 (272-I/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CCL1/I-309/TCA-3 (272-I/CF)

Discover more about diseases related to CCL1/I-309/TCA-3 (272-I/CF).
 

Pathways for CCL1/I-309/TCA-3 (272-I/CF)

View related products by pathway.

PTMs for CCL1/I-309/TCA-3 (272-I/CF)

Learn more about PTMs related to CCL1/I-309/TCA-3 (272-I/CF).

Blogs on CCL1/I-309/TCA-3

There are no specific blogs for CCL1/I-309/TCA-3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CCL1/I-309 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol CCL1
Entrez
Uniprot