Recombinant Human Calcium-sensing R/CaSR Protein, CF

• Reactivity: Hu

Recombinant Human Calcium-sensing R/CaSR Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived human Calcium-sensing R/CaSR protein
  Tyr20-Lys601, with a C-terminal 6-His tag
• Accession #: P41180-1
• N-terminal Sequence: Tyr20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Calcium-sensing R/CaSR Protein, CF

• calcium-sensing receptor
• CAR
• CaSR
• EIG8
• extracellular calcium-sensing receptor
• FHH
• FIH
• GPRC2A
• GPRC2AHHC
• HHC1
• hypocalciuric hypercalcemia 1
• NSHPT
• parathyroid Ca(2+)-sensing receptor 1
• Parathyroid cell calcium-sensing receptor
• PCaR1
• PCAR1MGC138441

Background

Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C‑terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.

1. Geng, Y. et al. (2016) Elife 5:e13662.
2. Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
3. Brown, E. M. et al. (1993) Nature 366:575.
4. Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.

Bioinformatics

• Uniprot:
  • P41180-1()