Recombinant Human Brorin/VWC2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Brorin/VWC2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When rhBrorin/VWC-2 is immobilized at 10 μg/mL (100 μL/well), the concentration of rhBMP-6 that produces 50% of the optimal binding response is found to be approximately 0.05-0.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Brorin/VWC2 protein
  Ser28-Met325, with an N-terminal 6-His tag
• Accession #: Q2TAL6
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: VWC2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 33.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 43 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Brorin/VWC2 Protein, CF

• Brain-specific chordin-like protein
• Brain-Specific Chordin-Like
• Brorin
• PSST739
• UNQ739
• Von Willebrand Factor C Domain Containing 2
• Von Willebrand Factor C Domain-Containing Protein 2
• VWC2

Background

Brorin (brain-specific chordin-like protein), also called VWC2, is an ~46 kDa glycoprotein that is a member of the Chordin family of secreted BMP regulators (1 ‑ 3). The human Brorin cDNA encodes 325 amino acids (aa) including a 27 aa signal sequence and a 298 aa secreted mature protein with two VWFC (von Willebrand factor C) domains. These domains contain a pattern of 10 cysteine residues that is conserved in other family members, with the remaining aa sequence sharing little identity (1). Human Brorin shares 90%, 91% and 95% aa sequence identity with mouse, rat and equine Brorin, respectively. It also shares aa identity with VWC2L (Brorin-like) of 37% overall and 62% within the VWFC domains (4). Brorin is predominantly expressed in embryonic and adult neural tissues in the mouse (1). Expression of Brorin mRNA is concentrated in neurons within the diencephalon and medulla oblongata, but is not detected in the developing cerebral cortex (1). Brorin binds and antagonizes BMPs, interacting via the VWFC domains (1 - 3). It promotes neurogenesis in mouse neural precursors (1). Knockdown of Brorin in zebrafish embryos results in morphological abnormalities in the brain and eye (1, 4).

1. Koike, N. et al. (2007) J. Biol. Chem. 282:15843.
2. Zhang, J-L. et al. (2007) J. Biol. Chem. 282:20002.
3. Fujisawa, T. et al. (2009) Biochem. Biophys. Res. Commun. 385:215.
4. Miwa, H. et al. (2009) FEBS Lett. 583:3643.

Bioinformatics

• Gene Symbol: VWC2
• Uniprot:
  • Q2TAL6(Human)