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Recombinant Human BMP-9 Protein

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Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.4-1.6 ng/mL.
1 μg/lane of Recombinant Human BMP-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 23 kDa, respectively.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      3209-BP
    • Availability
      Product Discontinued

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Recombinant Human BMP-9 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.4-1.6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein
Ser320-Arg429
Accession #
N-terminal Sequence
Ser320
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GDF2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
3209-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-9 Protein

  • BMP9
  • BMP-9
  • BMP9BMP-9Bone morphogenetic protein 9
  • GDF2
  • GDF-2
  • growth differentiation factor 2
  • growth/differentiation factor 2

Background

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4 - 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8 - 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
  4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
  5. Song, J.J. et al. (1995) Endocrinology 136:4293.
  6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
  7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
  8. Lopez-Coviella, I. et al. (2000) Science 289:313.
  9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
  10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
  11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
  12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
  13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.

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Publications for BMP-9 (3209-BP)(49)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(41)
Cell Culture
(4)
ELISA (Standard)
(1)
In Vivo
(2)
Surface Plasmon Resonance
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(37)
Mouse
(12)
Rat
(1)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using 3209-BP Applications Species
Baccouche, B;Lietuvninkas, L;Kazlauskas, A; Activin A Limits VEGF-Induced Permeability via VE-PTP International journal of molecular sciences 2023-05-12 [PMID: 37240047] (Bioassay, Human) Bioassay Human
M Morita, A Yoneda, N Tokunoh, T Masaki, K Shirakura, M Kinoshita, R Hashimoto, N Shigesada, J Takahashi, M Tachibana, S Tanaka, M Obana, N Hino, M Ikawa, K Tsujikawa, C Ono, Y Matsuura, H Kidoya, N Takakura, Y Kubota, T Doi, K Takayama, Y Yoshioka, Y Fujio, Y Okada Upregulation of Robo4 expression by SMAD signaling suppresses vascular permeability and mortality in endotoxemia and COVID-19 models Proceedings of the National Academy of Sciences of the United States of America, 2023-01-12;120(3):e2213317120. 2023-01-12 [PMID: 36634143] (Bioassay, Human) Bioassay Human
C Ivaldo, M Passalacqu, AL Furfaro, C d'Abramo, S Ruiz, PK Chatterjee, CN Metz, M Nitti, P Marambaud Oxidative stress-induced MMP- and gamma-secretase-dependent VE-cadherin processing is modulated by the proteasome and BMP9/10 Scientific Reports, 2023-01-11;13(1):597. 2023-01-11 [PMID: 36631513] (Cell Culture, Human) Cell Culture Human
Z Shonibare, M Monavarian, K O'Connell, D Altomare, A Shelton, S Mehta, R Jaskula-Sz, R Phaeton, MD Starr, R Whitaker, A Berchuck, AB Nixon, RC Arend, NY Lee, CR Miller, N Hempel, K Mythreye Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer Oncogene, 2022-07-26;40(4):111066. 2022-07-26 [PMID: 35905726] (Bioassay, Human) Bioassay Human
DL Marvin, L You, L Bornes, M van Dinthe, N Peters, H Dang, SK Hakuno, M Hornsveld, O Kranenburg, J van Rheene, JHT Rohling, MP Chien, P Ten Dijke, L Ritsma Dynamic Visualization of TGF-beta/SMAD3 Transcriptional Responses in Single Living Cells Cancers, 2022-05-19;14(10):. 2022-05-19 [PMID: 35626109] (Bioassay, Human) Bioassay Human
F Kawecki, J Jann, M Fortin, FA Auger, N Faucheux, J Fradette Preclinical Evaluation of BMP-9-Treated Human Bone-like Substitutes for Alveolar Ridge Preservation following Tooth Extraction International Journal of Molecular Sciences, 2022-03-18;23(6):. 2022-03-18 [PMID: 35328724] (Cell Culture, Human) Cell Culture Human
A Addante, C González-C, C Roncero, N Lazcanoitu, J García-Sáe, B Herrera, A Sánchez BMP9 Promotes an Epithelial Phenotype and a Hepatocyte-like Gene Expression Profile in Adult Hepatic Progenitor Cells Cells, 2022-01-21;11(3):. 2022-01-21 [PMID: 35159174] (Bioassay, Human) Bioassay Human
M Fujioka-Ko, SD Marjanowsk, M Kono, S Hino, N Saulacic, B Schaller Osteoinductive potential of recombinant BMP-9 in bone defects of mice treated with antiresorptive agents International journal of oral and maxillofacial surgery, 2021-08-26;0(0):. 2021-08-26 [PMID: 34454793] (Bioassay, Mouse) Bioassay Mouse
Q Jiang, Q Li, B Liu, G Li, G Riedemann, H Gaitantzi, K Breitkopf-, A Zeng, H Ding, K Xu BMP9 promotes methionine- and choline-deficient diet-induced nonalcoholic steatohepatitis in non-obese mice by enhancing NF-&amp;kapppaB dependent macrophage polarization International immunopharmacology, 2021-03-31;96(0):107591. 2021-03-31 [PMID: 33812253] (Bioassay, Mouse) Bioassay Mouse
B Tao, JR Kraehling, S Ghaffari, CM Ramirez, S Lee, JW Fowler, WL Lee, C Fernandez-, A Eichmann, WC Sessa BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells J Biol Chem, 2020-10-23;0(0):. 2020-10-23 [PMID: 33097593] (Bioassay, Human) Bioassay Human
Show All 49 Publications.

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Bioinformatics

Gene Symbol GDF2
Uniprot