Recombinant Human BMP-9 Protein

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1 μg/lane of Recombinant Human BMP-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 23 kDa, respectively.
Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.4-1.6 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-9 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.4-1.6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein
Ser320-Arg429
Accession #
N-terminal Sequence
Ser320
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GDF2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
3209-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-9 Protein

  • BMP9
  • BMP-9
  • BMP9BMP-9Bone morphogenetic protein 9
  • GDF2
  • GDF-2
  • growth differentiation factor 2
  • growth/differentiation factor 2

Background

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4 - 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8 - 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
  4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
  5. Song, J.J. et al. (1995) Endocrinology 136:4293.
  6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
  7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
  8. Lopez-Coviella, I. et al. (2000) Science 289:313.
  9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
  10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
  11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
  12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
  13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.

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3209-BP
Species: Hu
Applications: Bioactivity

Publications for BMP-9 (3209-BP)(37)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Standard), Enzyme Assay, In Vivo, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(28)
Cell Culture
(2)
ELISA (Standard)
(1)
Enzyme Assay
(1)
In Vivo
(2)
Surface Plasmon Resonance
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(27)
Mouse
(7)
Rat
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using 3209-BP Applications Species
Y Alinejad, MA Lauzon, G Grenier, F Balg, N Faucheux Both Human Hematoma Punctured from Pelvic Fractures and Serum Increase Muscle Resident Stem Cells Response to BMP9: A Multivariate Statistical Approach J Clin Med, 2020;9(4):. 2020 [PMID: 32325892] (Bioassay, Human) Bioassay Human
A Addante, C Roncero, N Lazcanoitu, R Méndez, L Almalé, M García-Álv, P Ten Dijke, I Fabregat, B Herrera, A Sánchez A Signaling Crosstalk between BMP9 and HGF/c-Met Regulates Mouse Adult Liver Progenitor Cell Survival Cells, 2020;9(3):. 2020 [PMID: 32204446] (Cell Culture, Human) Cell Culture Human
X Wang, L Zong, W Wang, J Yang, Y Xiang CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway J Cancer, 2020;11(2):272-283. 2020 [PMID: 31897223] (Cell Culture, Human) Cell Culture Human
L Yu, LA Dawson, M Yan, K Zimmel, YL Lin, CP Dolan, M Han, K Muneoka BMP9 stimulates joint regeneration at digit amputation wounds in mice Nat Commun, 2019;10(1):424. 2019 [PMID: 30723209] (In Vivo, Mouse) In Vivo Mouse
M Subileau, G Merdzhanov, D Ciais, V Collin-Fau, JJ Feige, S Bailly, D Vittet Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation Stem Cell Reports, 2018;0(0):. 2018 [PMID: 30595547] (Bioassay, Mouse) Bioassay Mouse
E Tillet, M Ouarné, A Desroches-, C Mallet, M Subileau, R Didier, A Lioutsko, G Belthier, JJ Feige, S Bailly A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29789425] (Western Blot) Western Blot
K Schlereth, D Weichenhan, T Bauer, T Heumann, E Giannakour, D Lipka, S Jaeger, M Schlesner, P Aloy, R Eils, C Plass, HG Augustin The transcriptomic and epigenetic map of vascular quiescence in the continuous lung endothelium Elife, 2018;7(0):. 2018 [PMID: 29749927] (Bioassay, Human) Bioassay Human
H Tian, JJ Huang, C Golzio, X Gao, M Hector-Gre, N Katsanis, GC Blobe Endoglin interacts with VEGFR2 to promote angiogenesis FASEB J., 2018;0(0):fj201700867RR. 2018 [PMID: 29401587] (Bioassay, Human) Bioassay Human
K Sakamoto, Y Kanematsu-, Y Kamada, M Oka, T Ohnishi, M Miwa, T Asami, H Inooka Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology Biochem Biophys Rep, 2017;11(0):33-39. 2017 [PMID: 28955765] (Bioassay, Human) Bioassay Human
SR Pasricha, PJ Lim, TL Duarte, C Casu, D Oosterhuis, K Mleczko-Sa, M Suciu, AR Da Silva, K Al-Hourani, J Arezes, K McHugh, S Gooding, JN Frost, K Wray, A Santos, G Porto, E Repapi, N Gray, SJ Draper, N Ashley, E Soilleux, P Olinga, MU Muckenthal, JR Hughes, S Rivella, TA Milne, AE Armitage, H Drakesmith Hepcidin is regulated by promoter-associated histone acetylation and HDAC3 Nat Commun, 2017;8(1):403. 2017 [PMID: 28864822] (Bioassay, Mouse) Bioassay Mouse
Show All 37 Publications.

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Bioinformatics

Gene Symbol GDF2
Uniprot