Recombinant Human BMP-9 Protein


1 μg/lane of Recombinant Human BMP-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 23 kDa, respectively.
Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.4-1.6 ng/mL.

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Applications Bioactivity

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Recombinant Human BMP-9 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.4-1.6 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
12.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
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3209-BP in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-9 Protein

  • BMP9
  • BMP-9
  • BMP9BMP-9Bone morphogenetic protein 9
  • GDF2
  • GDF-2
  • growth differentiation factor 2
  • growth/differentiation factor 2


Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4 - 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8 - 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
  4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
  5. Song, J.J. et al. (1995) Endocrinology 136:4293.
  6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
  7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
  8. Lopez-Coviella, I. et al. (2000) Science 289:313.
  9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
  10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
  11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
  12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
  13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.

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Publications for BMP-9 (3209-BP)(45)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Standard), Enzyme Assay, In Vivo, Surface Plasmon Resonance, Western Blot.

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Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using 3209-BP Applications Species
DL Marvin, L You, L Bornes, M van Dinthe, N Peters, H Dang, SK Hakuno, M Hornsveld, O Kranenburg, J van Rheene, JHT Rohling, MP Chien, P Ten Dijke, L Ritsma Dynamic Visualization of TGF-beta/SMAD3 Transcriptional Responses in Single Living Cells Cancers, 2022;14(10):. 2022 [PMID: 35626109] (Bioassay, Human) Bioassay Human
F Kawecki, J Jann, M Fortin, FA Auger, N Faucheux, J Fradette Preclinical Evaluation of BMP-9-Treated Human Bone-like Substitutes for Alveolar Ridge Preservation following Tooth Extraction International Journal of Molecular Sciences, 2022;23(6):. 2022 [PMID: 35328724] (Cell Culture, Human) Cell Culture Human
A Addante, C González-C, C Roncero, N Lazcanoitu, J García-Sáe, B Herrera, A Sánchez BMP9 Promotes an Epithelial Phenotype and a Hepatocyte-like Gene Expression Profile in Adult Hepatic Progenitor Cells Cells, 2022;11(3):. 2022 [PMID: 35159174] (Bioassay, Human) Bioassay Human
M Fujioka-Ko, SD Marjanowsk, M Kono, S Hino, N Saulacic, B Schaller Osteoinductive potential of recombinant BMP-9 in bone defects of mice treated with antiresorptive agents International journal of oral and maxillofacial surgery, 2021;0(0):. 2021 [PMID: 34454793] (Bioassay, Mouse) Bioassay Mouse
Q Jiang, Q Li, B Liu, G Li, G Riedemann, H Gaitantzi, K Breitkopf-, A Zeng, H Ding, K Xu BMP9 promotes methionine- and choline-deficient diet-induced nonalcoholic steatohepatitis in non-obese mice by enhancing NF-&amp;kapppaB dependent macrophage polarization International immunopharmacology, 2021;96(0):107591. 2021 [PMID: 33812253] (Bioassay, Mouse) Bioassay Mouse
B Tao, JR Kraehling, S Ghaffari, CM Ramirez, S Lee, JW Fowler, WL Lee, C Fernandez-, A Eichmann, WC Sessa BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells J Biol Chem, 2020;0(0):. 2020 [PMID: 33097593] (Bioassay, Human) Bioassay Human
Y Alinejad, MA Lauzon, G Grenier, F Balg, N Faucheux Both Human Hematoma Punctured from Pelvic Fractures and Serum Increase Muscle Resident Stem Cells Response to BMP9: A Multivariate Statistical Approach J Clin Med, 2020;9(4):. 2020 [PMID: 32325892] (Bioassay, Human) Bioassay Human
A Addante, C Roncero, N Lazcanoitu, R Méndez, L Almalé, M García-Álv, P Ten Dijke, I Fabregat, B Herrera, A Sánchez A Signaling Crosstalk between BMP9 and HGF/c-Met Regulates Mouse Adult Liver Progenitor Cell Survival Cells, 2020;9(3):. 2020 [PMID: 32204446] (Cell Culture, Human) Cell Culture Human
X Wang, L Zong, W Wang, J Yang, Y Xiang CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway J Cancer, 2020;11(2):272-283. 2020 [PMID: 31897223] (Cell Culture, Human) Cell Culture Human
G Drouin, V Couture, MA Lauzon, F Balg, N Faucheux, G Grenier Muscle injury-induced hypoxia alters the proliferation and differentiation potentials of muscle resident stromal cells Skelet Muscle, 2019;9(1):18. 2019 [PMID: 31217019] (Bioassay, Mouse) Bioassay Mouse
Show All 45 Publications.

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Gene Symbol GDF2