Recombinant Human BMP-3b/GDF-10 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-3b/GDF-10 Protein Summary

Details of Functionality
Bioassay data are not available.
Source
E. coli-derived human BMP-3b/GDF-10 protein
Gln369-Arg478, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Structure / Form
disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GDF10
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1543-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-3b/GDF-10 Protein

  • BIP
  • BMP3b
  • BMP-3b
  • bone morphogenetic protein 3B
  • Bone-inducing protein
  • GDF10
  • GDF-10
  • growth differentiation factor 10
  • Growth/differentiation factor 10

Background

BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta ) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta  family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2 - 4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).

  1. Miyazono, K. et al. (2001) J. Cell Physiol. 187:265.
  2. Cunningham, N.S. et al. (1995) Growth Factors 12:99.
  3. Takao, M. et al. (1996) Biochem. Biophys. Res. Comm. 219:656.
  4. Zhao, R. et al. (1999) Dev. Biol. 212:68.
  5. Hino, J. et al. (1999) Biochem.Biophys. Res. Comm. 256:419.
  6. Kaihara, S. et al. (2003) Life Sci. 72:1683.

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Publications for BMP-3b/GDF-10 (1543-BP)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Neural Differentiation, Surface Plasmon Resonance.


Filter By Application
Neural Differentiation
(1)
Surface Plasmon Resonance
(1)
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Filter By Species
Human
(2)
All Species

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Bioinformatics

Gene Symbol GDF10
Uniprot