Recombinant Human BMP-2/BMP-7 Heterodimer Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human BMP-2/BMP-7 Heterodimer Protein Summary

• Details of Functionality: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 15.0-120 ng/mL.
• Source: E. coli-derived human BMP-2/BMP-7 Heterodimer protein
  

  Human BMP-2 (Ala284 - Arg396) Accession # NP_001191

  Human BMP-7 (Ser293 - His431) Accession # NP_001710
  N-terminus	C-terminus

• Accession #: NP_001191 (BMP-2) & NP_001710 (BMP-7)
• N-terminal Sequence: Ala284 & Ser293
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: BMP2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12.9 kDa (BMP-2), 15.8 kDa (BMP-7).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as 0.2 μm filtered solution in 30%(v/v) Acetonitrile and 0.1%(v/v) TFA with 50 μg BSA per 1 μg as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

• BDA2;BMP2A;Bone morphogenetic protein 2;SSFSC;SSFSC1
• BMP-2/BMP-7 Heterodimer

Background

Human BMP-7, also known as osteogenic protein 1 (OP-1), and BMP-2 are members of the BMP subgroup of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-2 and BMP-7 influence a variety of morphogenic processes, particularly during skeletal and renal development (1 - 3). The human BMP-2 cDNA encodes a 396 amino acid (aa) precursor that contains a 23 aa signal sequence, a 259 propeptide, and a 114 aa mature protein (4). The human BMP-7 cDNA encodes a 431 aa precursor that contains a 29 aa signal sequence, a 263 aa propeptide, and a 139 aa mature protein (5). BMP propeptides are removed by proteolysis, enabling mature BMPs to form active disulfide linked homodimers or heterodimers (1). Human and mouse BMP-2 and BMP-7 are 100% and 98% identical, respectively, at the amino acid level. Human BMP-2 shares 85% aa sequence identity with human BMP-4 and less than 51% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60% - 70% aa sequence identity with BMP-5, -6, and -8, and less than 50% aa sequence identity with other BMPs. BMP-2 and BMP-7 are co-expressed in some embryonic tissues (6, 7) and associate into a functional 38 kDa osteogenic dimer (8). In in vitro osteoblast differentiation assays and in vivo bone formation models, a BMP-2/BMP-7 heterodimer is significantly more potent than either homodimer (9 - 12). Considering that BMP-2 preferentially binds BMPRIA/ALK-3 and BMPRIB/ALK-6, while BMP-7 is selective for ALK-2, the observed increase in heterodimer activity may be due to the triggering of additional receptor subtypes.

1. Chen, D. et al. (2004) Growth Factors 22:233.
2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
4. Wozney, J. et al. (1988) Science 242:1528.
5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
6. Lyons, K.M. et al. (1995) Mech. Dev. 50:71.
7. Knosp, W.M. et al. (2004) Development 131:4581.
8. Sampath, T.K. et al. (1990) J. Biol. Chem. 265:13198.
9. Hazama, M. et al. (1995) Biochem. Biophys. Res. Commun. 209:859.
10. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
11. Zhao, M. et al. (2005) J. Cell. Biochem. 95:1.
12. Israel, D.I. et al. (1996) Growth Fact ors 13:291.

Publications for BMP-2/BMP-7 Heterodimer (3229-BM)(17)

Publications using 3229-BM

• YS Yang, JM Kim, J Xie, S Chaugule, C Lin, H Ma, E Hsiao, J Hong, H Chun, EM Shore, FS Kaplan, G Gao, JH Shim Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery Oncogene, 2022-10-19;13(1):6175. 2022-10-19 [PMID: 36258013] (Bioassay, Human)
• H Greenfeld, J Lin, MC Mullins The BMP signaling gradient is interpreted through concentration thresholds in dorsal-ventral axial patterning PloS Biology, 2021-01-22;19(1):e3001059. 2021-01-22 [PMID: 33481775] (Bioassay, Zebrafish)
• JM Kim, YS Yang, KH Park, X Ge, R Xu, N Li, M Song, H Chun, S Bok, JF Charles, O Filhol-Coc, B Boldyreff, T Dinter, PB Yu, N Kon, W Gu, T Takarada, MB Greenblatt, JH Shim A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation Nat Commun, 2020-05-08;11(1):2289. 2020-05-08 [PMID: 32385263] (In Vivo, Mouse)
• A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration Int J Mol Sci, 2020-04-14;21(8):. 2020-04-14 [PMID: 32295299] (Bioassay, Human)
• WKW Ho, L Freem, D Zhao, KJ Painter, TE Woolley, EA Gaffney, MJ McGrew, A Tzika, MC Milinkovit, P Schneider, A Drusko, F Matthäus, JD Glover, KL Wells, JA Johansson, MG Davey, HM Sang, M Clinton, DJ Headon Feather arrays are patterned by interacting signalling and cell density waves PLoS Biol., 2019-02-21;17(2):e3000132. 2019-02-21 [PMID: 30789897] (Bioassay, Chicken)
• SEJ Tanis, ES Köksal, JAGL van Buggen, KW Mulder BLNCR is a long non-coding RNA adjacent to integrin beta-1 that is rapidly lost during epidermal progenitor cell differentiation Sci Rep, 2019-01-10;9(1):31. 2019-01-10 [PMID: 30631104] (Bioassay, Human)
• AM El-Naggar, PW Clarkson, GL Negri, B Turgu, F Zhang, MS Anglesio, PH Sorensen HACE1 is a potential tumor suppressor in osteosarcoma Cell Death Dis, 2019-01-08;10(1):21. 2019-01-08 [PMID: 30622235] (Bioassay, Human)
• SEJ Tanis, PWTC Jansen, H Zhou, SJ van Heerin, M Vermeulen, M Kretz, KW Mulder Splicing and Chromatin Factors Jointly Regulate Epidermal Differentiation Cell Rep, 2018-10-30;25(5):1292-1303.e5. 2018-10-30 [PMID: 30380419] (Bioassay, Human)
• W Bi, Y Liu, J Guo, Z Lin, J Liu, M Zhou, D Wismeijer, JL Pathak, G Wu All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity Cell Biosci, 2018-08-23;8(0):48. 2018-08-23 [PMID: 30159139] (Bioassay, Human)
• LD Loozen, A Vanderstee, AH Kragten, FC Öner, WJ Dhert, MC Kruyt, J Alblas Bone formation by heterodimers through non-viral gene delivery of BMP-2/6 and BMP-2/7 Eur Cell Mater, 2018-03-28;35(0):195-208. 2018-03-28 [PMID: 29589649] (ELISA (Standard))
• P Choudhary, H Booth, A Gutteridge, B Surmacz, I Louca, J Steer, J Kerby, PJ Whiting Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage Stem Cells Transl Med, 2016-08-24;6(2):490-501. 2016-08-24 [PMID: 28191760] (Bioassay, Human)
• Greenblatt M, Kim J, Oh H, Park K, Choo M, Sano Y, Tye C, Skobe Z, Davis R, Park J, Bei M, Glimcher L, Shim J p38alpha MAPK is required for tooth morphogenesis and enamel secretion. J Biol Chem, 2014-11-18;290(1):284-95. 2014-11-18 [PMID: 25406311] (Bioassay, Mouse)
• Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination. PLoS ONE, 2014-06-09;9(6):e99534. 2014-06-09 [PMID: 24911063] (Bioassay, Human)
• Goldie S, Mulder K, Tan D, Lyons S, Sims A, Watt F FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis. Cancer Res, 2012-05-07;72(13):3424-36. 2012-05-07 [PMID: 22564525] (Bioassay, Human)
• Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011-07-07;286(34):30034-46. 2011-07-07 [PMID: 21737454] (Surface Plasmon Resonance, Human, Mouse)
• Little SC, Mullins MC Bone morphogenetic protein heterodimers assemble heteromeric type I receptor complexes to pattern the dorsoventral axis. Nat. Cell Biol., 2009-04-19;11(5):637-43. 2009-04-19 [PMID: 19377468] (Bioassay, Zebrafish)
• Esterberg R, Delalande JM, Fritz A Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm. Development, 2008-10-23;135(23):3891-901. 2008-10-23 [PMID: 18948415] (In Vivo, Zebrafish)

Bioinformatics

• Gene Symbol: BMP2
• Uniprot:
  • NP_001191 (BMP-2) & NP_001710 (BMP-7)()