Recombinant Human ASAM Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ASAM Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 10⁴ cells/well are added to Recombinant Human ASAM coated plates (30 µg/mL, 100 µL/well), approximately 25%‑50% will adhere after one hour at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human ASAM protein
  Gly18-Met233, with a C-terminal 6-His tag
• Accession #: Q9H6B4
• N-terminal Sequence: Gly18
• Protein/Peptide Type: Recombinant Proteins
• Gene: CLMP
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 33-37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ASAM Protein, CF

• ACAM
• ACAMCoxsackie- and adenovirus receptor-like membrane protein
• Adipocyte adhesion molecule
• adipocyte-specific adhesion molecule
• ASAM
• ASAMCAR-like membrane protein
• Asp5
• CLMP
• CXADR-like membrane protein
• FLJ22415

Background

Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and coxsackie- and adenovirus receptor-like membrane protein (CLMP), is a type I transmembrane protein and member of the CTX family within the immunoglobulin superfamily. Mature human ASAM consists of a 217 amino acid (aa) extracellular region, a 21 aa transmembrane region, and a 117 aa cytoplasmic tail. The extracellular region contains one V-type and one C-type immunoglobulin domain connected by a J segment (1, 2). Within the ECD, human ASAM shares 97% aa sequence identity with mouse and rat ASAM. ASAM is widely expressed as 44 kDa and 48 kDa species in epithelial tissues where it localizes to tight junctions (1, 3). It mediates cell‑cell adhesion and contributes to trans-epithelial resistance (1). It is down‑regulated in Sertoli cells by TNF-alpha which also inhibits the expression of several other tight junction proteins in seminiferous tubules (4, 5). ASAM is additionally expressed by mature adipocytes and is up‑regulated in white adipose tissue under conditions of obesity (2).

1. Raschperger et al. (2004) J. Biol. Chem. 279:796.
2. Eguchi, J. et al. (2005) Biochem. J. 387:343.
3. Sze, K.-L. et al. (2008) J. Cell Physiol 214:334.
4. Sze, K.-L. et al. (2008) Biochem. J. 410:575.
5. Li, M.W. et al. (2006) J. Endocrinol. 190:313.

Bioinformatics

• Gene Symbol: CLMP
• Uniprot:
  • Q9H6B4()