Recombinant Human Annexin A3 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Annexin A3 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived human Annexin A3 protein
  Ala2-Asp323, with a C-terminal 6-His tag
• Accession #: P12429
• N-terminal Sequence: Ala2
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Annexin A3 Protein, CF

• 35-alpha calcimedin
• Annexin A3
• annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase
• Annexin III
• annexin-3
• ANXA3
• Calcimedin 35-alpha
• calcimedin 35-alpha
• Lipocortin III
• PAP-III
• Placental anticoagulant protein III
• placental anticoagulant protein III, calcimedin 35-alpha

Background

Annexin A3 (ANXA3), also known as Annexin III and Lipocortin III (LPC3), is a 37 kDa member of the Annexin family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (1). Annexin A3 is up-regulated in microglia following stroke and in parenchymal hepatocytes in regenerating liver (2, 3). It can be up- or down‑regulated in various carcinomas (4). Alternate splicing generates an additional isoform with a 30 amino acid (aa) deletion near the N-terminus (5). Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively.

1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
2. Kessler, C., et al. (2007). Rom. J. Morphol. Embryol. 49:27.
3. Harashima, M. et al. (2008). J. Biochem. 143:537.
4. Wu, N., et al. (2013). Clin. Transl. Oncol. 15:106.
5. Bianchi, C. et al. (2010). Am. J. Pathol. 176:1660.

Bioinformatics

• Uniprot:
  • P12429()