Recombinant Human Angiopoietin-like 4 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Inhibition Activity
Format
Carrier-Free

Order Details

Recombinant Human Angiopoietin-like 4 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770. The IC50 value under conditions in which Recombinant Human Lipoprotein Lipase/LPL (Catalog # 9888-LL)  and p-nitrophenyl butyrate are present in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is approximately 0.05-2.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
Gly26-Ser406 (Lys163Ala & Arg164Ala), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Gly26
Structure / Form
Oligomer
Protein/Peptide Type
Recombinant Proteins
Gene
ANGPTL4
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Inhibition Activity
Theoretical MW
43.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-55 kDa, reducing conditions
Publications
Read Publications using
4487-AN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 4 Protein, CF

  • Angiopoietin like Protein 4
  • angiopoietin-like 4
  • Angiopoietin-like Protein 4
  • ANGPTL4
  • ARP4fasting-induced adipose factor
  • FIAF
  • FIAFhepatic angiopoietin-related protein
  • Hepatic fibrinogen/angiopoietin-related protein
  • HFARP
  • HFARPANGPTL2
  • NL2
  • peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
  • PGAR
  • PGARangiopoietin-related protein 4
  • pp1158
  • PPARG angiopoietin related protein

Background

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature human ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT-L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins  beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).
  1. Zhu, P. et al. (2012) Biosci. Rep. 32:211.
  2. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
  3. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
  4. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
  5. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
  6. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
  7. Cazes, A. et al. (2006) Circ. Res. 99:1207.
  8. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
  9. Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
  10. Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
  11. Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
  12. Hou, M. et al. (2014) PLoS ONE 9:e85808.
  13. Clement, L.C. et al. (2011) Nat. Med. 17:117.

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Publications for Angiopoietin-like Protein 4/ANGPTL4 (4487-AN)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(1)
Bioassay
(10)
All Applications
Filter By Species
Human
(11)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 4487-AN Applications Species
J Huang, S Zhang Overexpressed Neuropilin-1 in Endothelial Cells Promotes Endothelial Permeability through Interaction with ANGPTL4 and VEGF in Kawasaki Disease Mediators of Inflammation, 2021;2021(0):9914071. 2021 [PMID: 34434074] (Bioassay, Human) Bioassay Human
M Li, J Hu, L Yao, M Gao Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32745373] (Bioassay, Human) Bioassay Human
M Li, J Hu, L Yao, M Gao Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32745373] (Bioassay, Human) Bioassay Human
YT Kang, WC Hsu, CC Ou, HC Tai, HT Hsu, KT Yeh, JL Ko Metformin Mitigates Nickel-Elicited Angiopoietin-Like Protein 4 Expression via HIF-1&amp;alpha for Lung Tumorigenesis Int J Mol Sci, 2020;21(2):. 2020 [PMID: 31963541] (Bioassay, Human) Bioassay Human
T Zhang, A Kastrenopo, Q Larrouture, NA Athanasou, HJ Knowles Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis BMC Cancer, 2018;18(1):536. 2018 [PMID: 29739381] (Bioassay, Human) Bioassay Human
K Baba, Y Kitajima, S Miyake, J Nakamura, K Wakiyama, H Sato, K Okuyama, H Kitagawa, T Tanaka, M Hiraki, K Yanagihara, H Noshiro Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines Sci Rep, 2017;7(1):11127. 2017 [PMID: 28894280] (Bioassay, Human) Bioassay Human
Contact of myeloma cells induces a characteristic transcriptome signature in skeletal precursor cells - Implications for myeloma bone disease Bone, 2016;0(0):. 2016 [PMID: 27519972] (Bioassay, Human) Bioassay Human
Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells. J Biol Chem, 2014;289(12):8402-12. 2014 [PMID: 24505142] (Bioassay, Human) Bioassay Human
Robal , Terje, Larsson , Mikael, Martin , Miina, Olivecrona , Gunilla, Lookene , Aivar Fatty acids bind tightly to the N-terminal domain of angiopoietin-like protein 4 and modulate its interaction with lipoprotein lipase. J Biol Chem, 2012;287(35):29739-52. 2012 [PMID: 22773878] (Binding Assay, Human) Binding Assay Human
Knowles HJ, Cleton-Jansen AM, Korsching E, Athanasou NA Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4. FASEB J., 2010;24(12):4648-59. 2010 [PMID: 20667978] (Bioassay, Human) Bioassay Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol ANGPTL4
Uniprot