Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF

• Reactivity: Hu
• Applications: Inhibition Activity
• Format: Carrier-Free

Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin‑like 4/ANGPTL4 C‑Terminal Fragment is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED₅₀ of 70.0-350 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
  Leu165-Ser406, with a C-terminal 6-His tag
• Accession #: Q9BY76
• N-terminal Sequence: Leu165
• Structure / Form: Oligomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ANGPTL4
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Inhibition Activity
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF

• Angiopoietin like Protein 4
• angiopoietin-like 4
• Angiopoietin-like Protein 4
• ANGPTL4
• ARP4fasting-induced adipose factor
• FIAF
• FIAFhepatic angiopoietin-related protein
• Hepatic fibrinogen/angiopoietin-related protein
• HFARP
• HFARPANGPTL2
• NL2
• peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
• PGAR
• PGARangiopoietin-related protein 4
• pp1158
• PPARG angiopoietin related protein

Background

ANGPT-L4, also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue that is structurally related to the angiopoietins. It contains an N‑terminal coiled coil domain and a C‑terminal fibrinogen-like domain which can be proteolytically separated in vivo (1, 2). Mature human ANGPT‑L4 shares 26%‑30% amino acid (aa) sequence identity with ANGPT-L1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT‑L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (3, 4). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (5-9). In human, the N-terminal fragment and full length ANGPT-L4 physically associate with HDL (9). In mouse, however, full length ANGPT-L4 associates with HDL, while the N-terminal fragment associates with LDL (9). Circulating ANGPT-L4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (10). Its expression in adipose tissue is induced by fasting and suppressed by feeding (3, 11). Its expression in both liver and fat is up‑regulated by PPAR alpha , beta , gamma , and δ agonists and down‑regulated by insulin (3, 12, 13). ANGPT-L4 is induced in vascular endothelial cells by hypoxia and in hypoxic areas surrounding tumors (14-16). The full length molecule but not the C-terminal fragment is bound by heparan sulfate proteoglycans and functions as an angiogenesis inhibitor (14, 15).

1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
2. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
3. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
4. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
5. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. 103:17450.
6. Koster, A. et al. (2005) Endocrinology 146:4943.
7. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770.
8. Ge, H. et al. (2004) J. Lipid Res. 45:2071.
9. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
10. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. 102:6086.
11. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
12. Mandard, S. et al. (2004) J. Biol. Chem. 279:34411.
13. Yamada, T. et al. (2006) Biochem. Biophys. Res. Commun. 347:1138.
14. Cazes, A. et al. (2006) Circ. Res. 99:1207.
15. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
16. Ito, Y. et al. (2003) Cancer Res. 63:6651.

Publications for Angiopoietin-like Protein 4/ANGPTL4 (3485-AN)(6)

Publications using 3485-AN

• Hefni, E;Menon, D;Ma, T;Asiedu, EB;Sultan, A;Meiller, T;Schneider, A;Sodhi, A;Montaner, S; Angiopoietin-like 4 induces head and neck squamous cell carcinoma cell migration through the NRP1/ABL1/PXN pathway Cellular signalling 2023-05-09 [PMID: 37169211] (Bioassay, Human)
• BW van der Ko, RG Vink, JWE Jocken, NJT Roumans, GH Goossens, ECM Mariman, MA van Baak, EE Blaak Effect of diet-induced weight loss on angiopoietin-like protein 4 and adipose tissue lipid metabolism in overweight and obese humans Physiol Rep, 2018-07-01;6(13):e13735. 2018-07-01 [PMID: 29998530] (ELISA (Standard), Human)
• EG Gruppen, S Kersten, RPF Dullaart Plasma angiopoietin-like 4 is related to phospholipid transfer protein activity in diabetic and non-diabetic subjects: role of enhanced low grade inflammation Lipids Health Dis, 2018-03-27;17(1):60. 2018-03-27 [PMID: 29587751] (ELISA (Standard))
• K Baba, Y Kitajima, S Miyake, J Nakamura, K Wakiyama, H Sato, K Okuyama, H Kitagawa, T Tanaka, M Hiraki, K Yanagihara, H Noshiro Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines Sci Rep, 2017-09-11;7(1):11127. 2017-09-11 [PMID: 28894280] (Bioassay, Human)
• Alex S, Lange K, Amolo T, Grinstead J, Haakonsson A, Szalowska E, Koppen A, Mudde K, Haenen D, Al-Lahham S, Roelofsen H, Houtman R, van der Burg B, Mandrup S, Bonvin A, Kalkhoven E, Muller M, Hooiveld G, Kersten S Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor gamma. Mol Cell Biol, 2013-01-22;33(7):1303-16. 2013-01-22 [PMID: 23339868] (ELISA (Standard), Human)
• Kersten S, Lichtenstein L, Steenbergen E, Mudde K, Hendriks HF, Hesselink MK, Schrauwen P, Muller M Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids. Arterioscler. Thromb. Vasc. Biol., 2009-04-02;29(6):969-74. 2009-04-02 [PMID: 19342599] (ELISA (Standard))

Bioinformatics

• Gene Symbol: ANGPTL4
• Uniprot:
  • Q9BY76()