Recombinant Human Angiopoietin-like 4 Biotin Protein, CF

Both Biotinylated Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # BT4487) and unlabeled Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN) promotes the expansion of E16-E18 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL). The ED50 for this effect is 100-600 ng/mL in the presence of a cross-linking antibody, His Tag MAb (Catalog # MAB050). The similarity in activity highlights that the biotinylated protein is fully functional.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Angiopoietin-like 4 Biotin Protein, CF Summary

• Details of Functionality: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 100-600 ng/mL in the presence of a cross-linking antibody, His Tag MAb, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
• Source: Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
  Gly26-Ser406 (Lys163Ala, Arg164Ala), with a C-terminal 6-His tag
• Accession #: Q9BY76
• N-terminal Sequence: Gly26
• Structure / Form: Oligomer. Biotinylated via sugars
• Protein/Peptide Type: Recombinant Proteins
• Gene: ANGPTL4
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 44 kDa (unlabeled).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 4 Biotin Protein, CF

• Angiopoietin like Protein 4
• angiopoietin-like 4
• Angiopoietin-like Protein 4
• ANGPTL4
• ARP4fasting-induced adipose factor
• FIAF
• FIAFhepatic angiopoietin-related protein
• Hepatic fibrinogen/angiopoietin-related protein
• HFARP
• HFARPANGPTL2
• NL2
• peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
• PGAR
• PGARangiopoietin-related protein 4
• pp1158
• PPARG angiopoietin related protein

Background

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature human ANGPTL4 shares 26% - 30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT-L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).

1. Zhu, P. et al. (2012) Biosci. Rep. 32:211.
2. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
3. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
4. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
5. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
6. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
7. Cazes, A. et al. (2006) Circ. Res. 99:1207.
8. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
9. Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
10. Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
11. Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
12. Hou, M. et al. (2014) PLoS ONE 9:e85808.
13. Clement, L.C. et al. (2011) Nat. Med. 17:117.

Bioinformatics

• Gene Symbol: ANGPTL4
• Uniprot:
  • Q9BY76()