Recombinant Human Angiopoietin-4 Protein


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Applications Binding Activity

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Recombinant Human Angiopoietin-4 Protein Summary

Details of Functionality
Measured by its ability to compete with biotinylated rhAng-2 for binding to immobilized Tie-2/Fc Chimera in a functional ELISA. Compared to rmAngiopoietin-3, rhAngiopoietin-4 is less effective in this competitive assay.
Mouse myeloma cell line, NS0-derived human Angiopoietin-4 protein
Human Ang-4
(Gln23 - Ile503) or (Gln24 - Ile503)
Accession # Q9Y264
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln23 or Gln24 predicted
Structure / Form
Protein/Peptide Type
Recombinant Proteins
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
57 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60-100 kDa, reducing conditions
Read Publications using
964-AN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-4 Protein

  • AGP4
  • ANG3
  • ANG-3
  • ANG-4
  • ANG4angiopoietin-3
  • angiopoietin 4
  • Angiopoietin-3
  • Angiopoietin-4
  • ANGPT4
  • dJ824F16.2 (angiopoietin 4)
  • MGC138181
  • MGC138183


Human Angiopoietin-4 (Ang-4) (1), alternatively named Ang-3 (2), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-4 cDNA encodes a 503 amino acid (aa) precursor protein with a 23 aa signal peptide. It shares 45%, 47% and 54% aa sequence identity with human Ang-1, human Ang-2 and mouse Ang-3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang-3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed multiple mouse tissues. Human Ang-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

  1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
  2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
  3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

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Publications for Angiopoietin-4 (964-AN)(6)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 4 applications: Bioassay, ELISA (Capture), Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.

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ELISA (Capture)
Surface Plasmon Resonance
Surface Plasmon Resonance (SPR
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Showing Publications 1 - 6 of 6.
Publications using 964-AN Applications Species
Q Hou, J Jia, J Lin, L Zhu, S Xie, Q Yu, Y Li Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection Cell Reports, 2022-09-27;40(13):111416. 2022-09-27 [PMID: 36170821] (Bioassay, Human) Bioassay Human
SB Mamer, A Wittenkell, PI Imoukhuede VEGF-A splice variants bind VEGFRs with differential affinities Sci Rep, 2020-09-02;10(1):14413. 2020-09-02 [PMID: 32879419] (Surface Plasmon Resonance, Surface Plasmon Resonance (SPR, Bioassay, Human, N/A) Surface Plasmon Resonance, Surface Plasmon Resonance (SPR, Bioassay Human, N/A
H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina Elife, 2018-11-16;7(0):. 2018-11-16 [PMID: 30444491] (Bioassay, ELISA (Capture), Surface Plasmon Resonance, Human) Bioassay, ELISA (Capture), Surface Plasmon Resonance Human
SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017-11-27;7(1):16439. 2017-11-27 [PMID: 29180757] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Brunckhorst M, Xu Y, Lu R, Yu Q Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment. Am J Pathol, 2014-08-01;184(8):2285-96. 2014-08-01 [PMID: 25043619] (Bioassay, Human) Bioassay Human
Brunckhorst MK, Wang H, Lu R, Yu Q Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis. Cancer Res., 2010-09-07;70(18):7283-93. 2010-09-07 [PMID: 20823154] (Bioassay, Human) Bioassay Human

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Gene Symbol ANGPT4