Recombinant Feline IL-12 Protein

Images

 
There are currently no images for IL-12 (1954-FL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity FeSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Feline IL-12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 1-5 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived feline IL-12 protein
Feline IL-12 p40
(Ile23 - Ser329) (Glu167Gly)
Accession # O02744
10-His tag
Feline IL-12 p35
(Arg26 - Ser222) (Ala221Ser)
Accession # O02743
10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg26 (p35) & Ile23 (p40)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL12A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
23.7 kDa (p35), 36.2 kDa (p40).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
31-33 kDa and 41-45 kDa, reducing conditions
Publications
Read Publications using
1954-FL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Feline IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12 (IL-12) is a 75 kDa heterodimeric glycoprotein that is composed of the disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits. Whereas p35 is an alpha -helical protein related to IL-6, the p40 subunit belongs to the type I cytokine receptor family. The feline IL-12 p35 gene encodes a 222 amino acids (aa) precursor protein with a 25 aa signal peptide. Mature feline p35 contains four potential N-linked glycosylation sites and one intrachain disulfide bond. p35 is synthesized constitutively but can be upregulated by the class II MHC-TCR interaction. p35 is never secreted unbound to p40. Mature feline p35 shows 91%, 88%, 86%, 57%, 56% and 75% aa identity with canine, human, porcine, rat, mouse and guinea pig p35, respectively. The feline p40 gene encodes a secreted 329 aa precursor protein with a 22 aa signal peptide. Mature feline p40 contains one fibronectin type III and one Ig C2-like domain. It is known to exist as a monomer, a homodimer, and as heterodimers linked either to p35 to form IL-12, or to p19 to create IL-23. p40 synthesis is upregulated by many different inflammatory signals including LPS and CpG. Mature feline p40 shows 94%, 85%, 85%, 67%, 67% and 76% aa identity to canine, human, porcine, rat, mouse and guinea pig p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The signaling receptor for IL-12 is composed of two type I transmembrane glycoproteins belonging to the type I cytokine receptor superfamily. IL-12 receptor beta 1 (IL-12 R beta 1) is 100 kDa protein that serves as the principal binding site for the p40 subunit. IL-12 receptor beta 2 (IL-12 R beta 2) is 130 kDa protein that interacts primarily with p35 and functions as the signal transducing component. IL-12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, promotes IgG production (in mice), and potentiates the expansion and late activation of Th1 CD4+ T cells (1 - 5).

  1. Schijns, V.E. et al. (1997) Immunogenetics 45:462.
  2. Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
  3. Trinchieri, G. et al. (2003) Immunity 19:641.
  4. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  5. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.

Customers Who Viewed This Item Also Viewed...

NBP2-15843
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF1916
Species: Hu
Applications: WB, IHC, ICC
409-ML/CF
Species: Mu
NBP2-21577
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
NBP1-84022
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81796
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32956
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-67117
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-38780
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF7250
Species: Hu
Applications: IHC
NBP1-49833
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
AF1857
Species: Mu
Applications: WB, Simple Western, IHC, IP
NBP1-31650
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KO

Publications for IL-12 (1954-FL)(2)

Reviews for IL-12 (1954-FL) (0)

There are no reviews for IL-12 (1954-FL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-12 (1954-FL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-12 Products

Bioinformatics Tool for IL-12 (1954-FL)

Discover related pathways, diseases and genes to IL-12 (1954-FL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-12 (1954-FL)

Discover more about diseases related to IL-12 (1954-FL).
 

Pathways for IL-12 (1954-FL)

View related products by pathway.

PTMs for IL-12 (1954-FL)

Learn more about PTMs related to IL-12 (1954-FL).

Blogs on IL-12.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Ly6G - a marker for monocytes, granulocytes and neutrophils
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes expres...  Read full blog post.

Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Feline IL-12 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol IL12A
Uniprot