Recombinant Feline IL-12 Protein


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Recombinant Feline IL-12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 1-5 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived feline IL-12 protein
Feline IL-12 p40
(Ile23 - Ser329) (Glu167Gly)
Accession # O02744
10-His tag
Feline IL-12 p35
(Arg26 - Ser222) (Ala221Ser)
Accession # O02743
10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg26 (p35) & Ile23 (p40)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
23.7 kDa (p35), 36.2 kDa (p40).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
31-33 kDa and 41-45 kDa, reducing conditions
Read Publications using
1954-FL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Feline IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35


Interleukin 12 (IL-12) is a 75 kDa heterodimeric glycoprotein that is composed of the disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits. Whereas p35 is an alpha -helical protein related to IL-6, the p40 subunit belongs to the type I cytokine receptor family. The feline IL-12 p35 gene encodes a 222 amino acids (aa) precursor protein with a 25 aa signal peptide. Mature feline p35 contains four potential N-linked glycosylation sites and one intrachain disulfide bond. p35 is synthesized constitutively but can be upregulated by the class II MHC-TCR interaction. p35 is never secreted unbound to p40. Mature feline p35 shows 91%, 88%, 86%, 57%, 56% and 75% aa identity with canine, human, porcine, rat, mouse and guinea pig p35, respectively. The feline p40 gene encodes a secreted 329 aa precursor protein with a 22 aa signal peptide. Mature feline p40 contains one fibronectin type III and one Ig C2-like domain. It is known to exist as a monomer, a homodimer, and as heterodimers linked either to p35 to form IL-12, or to p19 to create IL-23. p40 synthesis is upregulated by many different inflammatory signals including LPS and CpG. Mature feline p40 shows 94%, 85%, 85%, 67%, 67% and 76% aa identity to canine, human, porcine, rat, mouse and guinea pig p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The signaling receptor for IL-12 is composed of two type I transmembrane glycoproteins belonging to the type I cytokine receptor superfamily. IL-12 receptor beta 1 (IL-12 R beta 1) is 100 kDa protein that serves as the principal binding site for the p40 subunit. IL-12 receptor beta 2 (IL-12 R beta 2) is 130 kDa protein that interacts primarily with p35 and functions as the signal transducing component. IL-12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, promotes IgG production (in mice), and potentiates the expansion and late activation of Th1 CD4+ T cells (1 - 5).

  1. Schijns, V.E. et al. (1997) Immunogenetics 45:462.
  2. Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
  3. Trinchieri, G. et al. (2003) Immunity 19:641.
  4. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  5. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.

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Gene Symbol IL12A