Recombinant Equine FGF basic/FGF2/bFGF Protein, CF

• Reactivity: Eq
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Equine FGF basic/FGF2/bFGF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.2-1 ng/mL.
• Source: E. coli-derived equine FGF basic/FGF2/bFGF protein
  Pro10-Ser155
• Accession #: E0AEV7
• N-terminal Sequence: Pro10 & Ala11
• Protein/Peptide Type: Recombinant Proteins
• Gene: FGF2
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, TCEP and EDTA.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Equine FGF basic/FGF2/bFGF Protein, CF

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

FGF basic, also known as FGF-2 or HBGF-2, is a widely expressed member of the fibroblast growth factor family (1-3). FGF basic functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain (2, 4-6). It is up-regulated in response to inflammatory stimuli such as TNF-alpha , IL-1 beta , IL-2, PDGF, and nitric oxide (2). The up-regulation of FGF basic in many human tumors is associated with tumor vascularity (2, 3). Among FGF family members, only FGF acidic and FGF basic lack signal peptides and are secreted by an alternative pathway. FGF basic is additionally localized in the nucleus where it regulates gene expression (2, 7). In humans, the use of alternative start sites can produce 21-23 kDa isoforms found only in the nucleus, and these isoforms target the expression of distinct genes (7). FGF family proteins share 35-60% amino acid (aa) identity. The 18 kDa equine FGF basic shares 97-99% amino acid  identity with bovine, canine, human, mouse, and rat FGF basic (8). FGF basic binds with picomolar affinity to FGF R1c and 2c (1, 2). Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha v beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen,
alpha 2-Macroglobulin, PDGF, and CXCL4/PF4 (2). These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones (2). In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors (1, 2).

1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
2. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
3. Grose, R. and C. Dickson (2005) Cytokine Growth Factor Rev. 16:179.
4. Rosenblatt-Velin, N. et al. (2005) J. Clin. Invest. 115:1724.
5. Reuss, B. et al. (2003) Cell Tissue Res. 313:139.
6. Su, N. et al. (2008) Front. Biosci. 13:2842.
7. Claus, P. et al. (2003) J. Biol. Chem. 278:479.
8. Abraham, J.A. et al. (1986) Science 233:545.

Publications for FGF basic/FGF2/bFGF (8577-FB/CF)(1)

Publication using 8577-FB/CF

• ALF Longhini, TE Salazar, C Vieira, T Trinh, Y Duan, LM Pay, S Li Calzi, M Losh, NA Johnston, H Xie, M Kim, RJ Hunt, MC Yoder, D Santoro, TM McCarrel, MB Grant Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses PLoS ONE, 2019-03-14;14(3):e0212642. 2019-03-14 [PMID: 30870461] (Bioassay, Equine)

FAQs for FGF basic/FGF2/bFGF (8577-FB/CF). (Showing 1 - 1 of 1 FAQ).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2
• Uniprot:
  • E0AEV7()