Recombinant Cynomolgus TNF RI/TNFRSF1A Fc Protein, CF

Images

 
Recombinant Cynomolgus Monkey TNF RI/TNFRSF1A Fc Chimera(Catalog # 9884-TR) inhibits Recombinant Human TNF‑ alpha (Catalog # 210-TA) mediated cytotoxicity in the L‑929 mouse fibroblast cells. The ED50 for this ...read more
2 μg/lane of Recombinant Cynomolgus Monkey TNF RI/TNFRSF1A was resolved with SDS-PAGEunder reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-66 kDa and ...read more

Product Details

Summary
Reactivity CmSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Cynomolgus TNF RI/TNFRSF1A Fc Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.6-3.6 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey TNF RI/TNFRSF1A protein
Cynomolgus Monkey TNF RI/TNFRSF1A
(Leu30-Thr211)
Accession # NP_001306550
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus
C-terminus
Accession #
N-terminal Sequence
Leu30
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-66 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus TNF RI/TNFRSF1A Fc Protein, CF

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF receptor 1 is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). TNF RI is a 455 amino acid (aa) protein that contains a signal sequence and ECD with a PLAD (pre-ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a transmembrane domain, and a cytoplasmic domain that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of cynomolgus TNF RI shows 97%, 69%, and 68% aa identity with human, mouse and rat TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). TNF RI is essential for proper development of lymph node germinal centers and Peyer's patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF-beta/TNFSF1B (lymphotoxin-alpha) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro‑inflammatory stimuli (7). TNF-alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8, 9, 16). Release of the 28-34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10-12). Full-length TNF RI may also be released in exosome-like vesicles (13). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (10, 14-15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR-associated periodic syndrome) (7).
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Chan, F.K. et al. (2000) Science 288:2351.
  4. Schall, T.J. et al. (1990) Cell 61:361.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  6. Banner, D.W et al. (1993) Cell 73:431.
  7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
  8. Legler, D.F. et al. (2003) Immunity 18:655.
  9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  11. Jin, L. et al. (2000) J. Immunol. 165:5153.
  12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  15. McDermott, M.F. et al. (1999) Cell 97:133.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
H00001523-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
MAB224
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
MAB3468
Species: Hu
Applications: WB, ICC
H00003059-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, PLA, RNAi
NBP1-18910
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1138
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
7270-IL/CF
Species: Hu
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NB100-56715
Species: Hu
Applications: WB, IHC, IHC-P

Publications for TNF RI/TNFRSF1A (9884-TR) (0)

There are no publications for TNF RI/TNFRSF1A (9884-TR).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TNF RI/TNFRSF1A (9884-TR) (0)

There are no reviews for TNF RI/TNFRSF1A (9884-TR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for TNF RI/TNFRSF1A (9884-TR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Cynomolgus TNF RI/TNFRSF1A Fc Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot