Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (AVI1300) binds with an ED50 of 25.0-200 ng/mL.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-65 kDa and 90-130 kDa, respectively.

• Reactivity: Pm-Cm, RM
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera (Catalog # 10960-NK) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (Catalog # AVI1300) binds with an ED₅₀ of 25.0‑200 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived NKG2D/CD314 protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Cynomologus Monkey NKG2D (Trp74-Val216) Accession # P61252.1
  N-terminus			C-terminus

• Accession #: P61252.1
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-65 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

• CD314 antigen
• CD314
• D12S2489E
• FLJ17759
• FLJ75772
• Killer cell lectin-like receptor subfamily K member 1
• killer cell lectin-like receptor subfamily K, member 1
• KLR
• KLRK1
• NK cell receptor D
• NKG2-D type II integral membrane protein
• NKG2D
• NKG2-D
• NKG2-D-activating NK receptor
• NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

Natural killer group 2, member D (NKG2D) is a type II lectin-like transmembrane glycoprotein is an activating receptor involved in both innate and adaptive immunities. Cynomologus NKG2D consists of an extracellular domain (ECD) with a C-type lectin domain, a transmembrane segment and a short cytoplasmic domain lacking signaling properties. The ECD lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands (1). The ECD of cynomologus NKG2D shares 98% amino acid sequence identity with the ECD of human NKG2D. NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells and NKT cells and additionally occurs on macrophages in mouse (1 - 3). The NKG2D receptor consists of a homodimer and has been shown to mediate immune responses and determine the activation status of NK cells (4). In human, ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3 (3, 5). Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection (6, 7). Alternative splicing of the NKG2D results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal (7). NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection (6, 8).

1. Li, P. et al. (2001) Nature Immunol. 2:443.
2. Steinle, A. et al. (2001) Immunogenetics 53:279.
3. Wang, J. et al. (2020) Biomol. 10:301.
4. Garrity, D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:7641.
5. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
8. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.

Bioinformatics

• Uniprot:
  • P61252.1()