Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF

When Recombinant Human Nectin-3 (Catalog # 3064-N3) is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED50 of 2.5-20 ng/mL.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 87-100 kDa and 170-200 kDa, respectively.

• Reactivity: Pm-Cm, RM
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF Summary

• Additional Information: Fc Chimera
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 His-tag (Catalog # 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED₅₀ of 2.5-20 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived Nectin-1 protein
  

  Cynomolgus Monkey/Rhesus Macaque Nectin-1 (Gln31-Gly349) Accession # XP_005579992.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: XP_005579992.1
• N-terminal Sequence: No results obtained: Gln31 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 87-101 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF

• CD111 antigen
• CD111
• CLPED1ectodermal dysplasia 4 (Margarita Island type)
• ED4
• Herpes virus entry mediator C
• Herpesvirus entry mediator C
• Herpesvirus Ig-like receptor
• HIgRPVRR
• HVEC
• HVECpoliovirus receptor-like 1
• MGC142031
• Nectin1
• Nectin-1
• OFC7nectin 1
• poliovirus receptor-related 1 (herpesvirus entry mediator C)
• poliovirus receptor-related protein 1
• PRR
• PRR1
• PRR1MGC16207
• PVRL1
• PVRR1
• PVRR1nectin-1
• SK-12

Background

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means "to connect", indicating the role of nectins in Ca²⁺-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). Based on the similarity with human Nectin-1, the 519 amino acid (aa) cynomolgus Nectin-1 may contain a 30 aa signal sequence, a 328 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding, and two C2-type (3,4). The ECD of cynomolgus Nectin-1 shares 98% and 100% aa identity with human and rhesus monkey Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14). In colorectal cancer, Nectin-1 expression was found to be associated with a high risk for early disease recurrence (15).

1. Lopez, M. et al. (1995) Gene 155:261.
2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
4. Lopez, M. et al. (2001) J. Virol. 75:5684.
5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.
7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.
10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.
14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
15. Tampakis, A. et al. (2019) Oncology. 96:318.

Bioinformatics

• Uniprot:
  • XP_005579992.1()