Recombinant Chicken Netrin-1 Protein

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Summary
Reactivity ChSpecies Glossary
Applications Binding Activity

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Recombinant Chicken Netrin-1 Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rcNetrin-1 with a linear range of 6-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived chicken Netrin-1 protein
Chicken Netrin-1
(Gly26 - Ala606)
Accession # Q90922
DIEGRGGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly26
Protein/Peptide Type
Recombinant Proteins
Gene
NTN1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
66.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-85 kDa, reducing conditions
Publications
Read Publications using
128-N1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Chicken Netrin-1 Protein

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of

Background

Chicken Netrin-1 is the prototypical member of an ever-expanding, laminin-related family of axon-guidance molecules collectively referrred to as netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 606 amino acid (aa) protein precursor that has structural similarity to the N-terminus of the B2 or gamma -chain of laminin. It contains one 250 aa type VI globular domain, three type V (~55 aa) cysteine/glycine rich EGF repeats, and one unique 140 aa “C” domain that binds heparin. Chick Netrin-1 shares 78% aa identity with chicken Netrin-2 and 86% aa identity with mouse and human Netrin-1. Although only two chick netrins are known, the number of known mammalian netrins is increasing. Human and mouse Netrin-3/NTN-2L, and a mouse Netrin-4, that shares homology with the B1 or beta -chain of laminin, have been reported. Cells reported to express Netrin-1 in the embryo include cells of the spinal cord floor plate and somite, cells of the ganglionic eminence, and cells of the floor plate of the met- and caudal mesencephalon. In the adult, neurons of the thalamus, neocortex, and hippocampus, plus Schwann cells, osteoclasts and osteoblasts all reportedly produce Netrin-1. The DCC (deleted in colorectal cancer) gene product as well as the UNC5 family of receptors and the adenosine A2b receptor have been proposed to be functional receptors for Netrin-1.

  1. Serafini, T. et al. (1994) Cell 78:409.
  2. Kennedy, T.E. et al. (1994) Cell 78:425.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. MacLennan, A.J. et al. (1997) J. Neurosci. 17:5466.
  5. Koch, M. et al. (2000) J. Cell. Biol. 151:221.
  6. Corset, V. et al. (2000) Nature 407:747.

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Publications for Netrin-1 (128-N1)(8)

We have publications tested in 4 confirmed species: Human, Rat, Chicken, Hamster.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(7)
In Vivo
(1)
All Applications
Filter By Species
Human
(3)
Rat
(2)
Chicken
(2)
Hamster
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 128-N1 Applications Species
CY Huang, CC Lien, CF Cheng, TY Yen, CJ Chen, ML Tsaur K(+) channel Kv3.4 is essential for axon growth by limiting the influx of Ca(2+) into growth cones J. Neurosci, 2017;0(0):. 2017 [PMID: 28320840] (Bioassay, Chicken) Bioassay Chicken
CD151 mediates netrin-1-induced angiogenesis through the Src-FAK-Paxillin pathway J Cell Mol Med, 2016;0(0):. 2016 [PMID: 27558487] (In Vivo, Rat) In Vivo Rat
Li J, Duarte T, Kocabas A, Works M, McConnell S, Hynes M Evidence for topographic guidance of dopaminergic axons by differential Netrin-1 expression in the striatum. Mol Cell Neurosci, 2014;61(0):85-96. 2014 [PMID: 24867253] (Bioassay, Rat) Bioassay Rat
van Gils , Janine M, Ramkhelawon , Bhama, Fernandes , Luciana, Stewart , Merran C, Guo , Liang, Seibert , Tara, Menezes , Gustavo, Cara , Denise C, Chow , Camille, Kinane , T Bernar, Fisher , Edward A, Balcells , Mercedes, Alvarez-Leite , Jacqueli, Lacy-Hulbert , Adam, Moore , Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013;33(5):911-9. 2013 [PMID: 23430612] (Bioassay, Human) Bioassay Human
Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc. Natl. Acad. Sci. U.S.A., 2008;105(34):12491-6. 2008 [PMID: 18719102] (Bioassay, Human) Bioassay Human
Hutchins BI, Kalil K Differential outgrowth of axons and their branches is regulated by localized calcium transients. J. Neurosci., 2008;28(1):143-53. 2008 [PMID: 18171932] (Bioassay, Hamster) Bioassay Hamster
Ly NP, Komatsuzaki K, Fraser IP, Tseng AA, Prodhan P, Moore KJ, Kinane TB Netrin-1 inhibits leukocyte migration in vitro and in vivo. Proc. Natl. Acad. Sci. U.S.A., 2005;102(41):14729-34. 2005 [PMID: 16203981] (Bioassay, Human) Bioassay Human
Tsai HH, Tessier-Lavigne M, Miller RH Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Development, 2003;130(10):2095-105. 2003 [PMID: 12668624] (Bioassay, Chicken) Bioassay Chicken

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Bioinformatics

Gene Symbol NTN1
Uniprot