Recombinant Canine CD25/IL-2R alpha Protein, CF

• Reactivity: Ca
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Canine CD25/IL-2R alpha Protein, CF Summary

• Details of Functionality: Measured by its ability to block IL-2-induced proliferation of CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.125-0.75 µg/mL in the presence of 2 ng/mL of Recombinant Canine IL‑2 (Cys147Ser) (Catalog # 1815‑CL).
• Source: Mouse myeloma cell line, NS0-derived canine CD25/IL-2 R alpha protein
  Met1-Ile238 with a C-terminal 6-His tag
• Accession #: BAI49682
• N-terminal Sequence: Tyr19 & Thr21
• Structure / Form: Noncovalently-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL2RA
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine CD25/IL-2R alpha Protein, CF

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c). IL‑2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3).  The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 20 aa signal peptide, a 217 aa extracellular domain (ECD) with two Sushi domains, a 19 aa transmembrane segment, and an 12 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49%-60% aa sequence identity with human, mouse, and rat IL-2 R alpha . IL-2 R beta (CD122) and gamma _c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma _c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (11-13).

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67.
3. Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638.
4. Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137.
5. Hatakeyama, M. et al. (1989) Science 244:551.
6. Takeshita, T. et al. (1992) Science 257:379.
7. Wang, X. et al. (2005) Science 310:1159.
8. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
9. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
11. Willerford, D.M. et al. (1995) Immunity 3:521.
12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • BAI49682()