Recombinant Amphibian TGF-beta 5 Protein, CF

• Reactivity: Am
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Amphibian TGF-beta 5 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED₅₀ for this effect is 0.01-0.03 ng/mL.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived amphibian TGF-beta 5 protein
  Gly271 -Ser382 & Gln274-Ser382
• Accession #: P16176
• N-terminal Sequence:

  Gly271 & Gln274

• Protein/Peptide Type: Recombinant Proteins
• Gene: tgfb1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions:

  Reconstitute at 20 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.

Notes

Triton is a registered trademark of Union Carbide Corp.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Amphibian TGF-beta 5 Protein, CF

• CED;DPD1;IBDIMDE;LAP;TGFB;TGFB1;TGFbeta;TGF-beta1;Transforming growth factor beta;Transforming growth factor beta-1 proprotein
• TGFbeta 5
• TGF-beta 5

Background

TGF-beta 5 is a member of the TGF-beta family of growth factors. These proteins are stable, multifunctional factors with a wide variety of effects on the growth and differentiation of virtually all cell types. These actions on growth or differentiation may be stimulatory or inhibitory, depending on the cell type, growth conditions, state of differentiation and on the presence of other growth factors. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. The in vivo role of TGF-beta 5 is also not known, but the presence of this factor in Xenopus embryos, tadpole cells, and adult tissues suggests a role in the mediation of differentiation and development. Similar roles have been suggested for other members of the TGF-beta family. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

Publications for TGF-beta 5 (245-B5/CF)(1)

Publication using 245-B5/CF

• Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein. Int. J. Dev. Biol., 2003-02-01;47(1):15-29. 2003-02-01 [PMID: 12653248] (In Vivo, Xenopus)

Bioinformatics

• Gene Symbol: tgfb1
• Uniprot:
  • P16176()