RECK Antibody (198212)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 198212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

RECK Antibody (198212) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human RECK
  Ala29-Val934
  Accession # O95980
• Specificity: Detects human RECK in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RECK
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RECK Antibody (198212)

• hRECK
• membrane-anchored glycoprotein (metastasis and invasion)
• RECK
• reversion-inducing-cysteine-rich protein with kazal motifs
• ST15
• ST15reversion-inducing cysteine-rich protein with Kazal motifs
• suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein withkazal motifs)
• suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein withkazal motifs)
• Suppressor of tumorigenicity 15 protein

Background

RECK is a GPI-anchored glycoprotein expressed in many normal tissues and down-regulated in many tumors and cells transformed by various oncogenes. It acts via a post-transcriptional event to decrease secretion of MMP-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RECK Antibody (MAB1734)(1)

Publication using MAB1734

• Jeon HW, Lee YM Inhibition of histone deacetylase attenuates hypoxia-induced migration and invasion of cancer cells via the restoration of RECK expression. Mol. Cancer Ther., 2010-05-04;9(5):1361-70. 2010-05-04 [PMID: 20442303] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RECK
• Uniprot:
  • O95980()