RDH14 Antibody - BSA Free

Western Blot: RDH14 Antibody [NBP3-06302] - Anti-RDH14 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLane B: PC3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: RDH14 Antibody [NBP3-06302] - Immunochemical staining of human RDH14 in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: RDH14 Antibody [NBP3-06302] - Immunochemical staining of human RDH14 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: RDH14 Antibody [NBP3-06302] - RDH14 was immunoprecipitated using:Lane A:0.5 mg HEK293 Whole Cell Lysate4 uL anti-RDH14 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-RDH14 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 37 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RDH14 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human RDH14 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RDH14
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for RDH14 Antibody - BSA Free

• Alcohol dehydrogenase PAN2
• EC 1.1.1.-
• PAN2retinol dehydrogenase 14
• pancreas protein 2
• retinol dehydrogenase 14 (all-trans and 9-cis)
• retinol dehydrogenase 14 (all-trans/9-cis/11-cis)
• SDR7C4
• short chain dehydrogenase/reductase family 7C, member 4

Background

Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent retinalreductase. Displays high activity toward 9-cis and all-trans-retinol. No steroid dehydrogenase activity detected

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RDH14