RBX1 Antibody - BSA Free

Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.

Western Blot: RBX1 Antibody [NBP2-20113] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RBX1 antibody (NBP2-20113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Ze
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RBX1 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RBX1. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Rat (100%), Chicken (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RBX1 Antibody - BSA Free

• BA554C12.1
• EC 6.3.2.-
• FLJ60363
• MGC13357
• Protein ZYP
• RBX1
• Regulator of cullins 1
• Ring Box 1
• RING box protein 1
• RING finger protein 75
• ring-box 1
• ring-box 1, E3 ubiquitin protein ligase
• RING-box protein 1
• RNF75
• RNF75MGC1481
• ROC1E3 ubiquitin-protein ligase RBX1
• ZYP Protein

Background

This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RBX1 Antibody (NBP2-20113)(1)

Publication using NBP2-20113

• Vava A, Paccez JD, Wang Y et al. DCUN1D1 Is an Essential Regulator of Prostate Cancer Proliferation and Tumour Growth That Acts through Neddylation of Cullin 1, 3, 4A and 5 and Deregulation of Wnt/Catenin Pathway Cells 2023-07-31 [PMID: 37566052] (Western Blot)

Bioinformatics

• Gene Symbol: RBX1
• Uniprot:
  • P62877(Human)