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HAF018). A specific band was detected for RBX1 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
ROC1 is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.
![Western Blot RBX1 Antibody (1076128) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11499_human-cul1-rbx1-complex-mab-clone-1076128-western-blot-283202473956..jpg)
![Immunohistochemistry RBX1 Antibody (1076128) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11499_human-cul1-rbx1-complex-mab-clone-1076128-immunohistochemistry-2032024125135..jpg)
![Immunocytochemistry RBX1 Antibody (1076128) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11499_human-cul1-rbx1-complex-mab-clone-1076128-immunocytochemistry-2032024125634..jpg)
![Simple Western RBX1 Antibody (1076128) [Unconjugated]](http://images.novusbio.com/fullsize/antibody/mab11499_human-cul1-rbx1-complex-mab-clone-1076128-simple-western-283202474839.jpg)
