RBPMS Antibody (OTI3B7)

Western Blot: RBPMS Antibody (3B7) [NBP2-03905] Analysis of extracts (35ug) from 9 different cell lines by using anti-RBPMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Human tonsil.

Flow Cytometry: RBPMS Antibody (3B7) [NBP2-03905] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RBPMS antibody, and then analyzed by flow cytometry.

Western Blot: RBPMS Antibody (3B7) [NBP2-03905] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBPMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBPMS.

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RBPMS mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RBPMS mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RBPMS mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Staining of paraffin-embedded Human Kidney tissue using anti-RBPMS mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Staining of paraffin-embedded Human lung tissue using anti-RBPMS mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Staining of paraffin-embedded Human pancreas tissue using anti-RBPMS mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody (3B7) [NBP2-03905] - Staining of paraffin-embedded Human prostate tissue using anti-RBPMS mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, IHC
• Clone: OTI3B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RBPMS Antibody (OTI3B7) Summary

• Immunogen: Full length human recombinant protein of human RBPMS (NP_001008712) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBPMS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 24.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 29954626). Mouse reactivity reported in scientific literature (PMID: 30073365). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBPMS Antibody (OTI3B7)

• FLJ32971
• Heart and RRM expressed sequence
• HERMES
• HERMESRNA-binding protein with multiple splicing
• RBPMS
• RBP-MS
• RNA binding protein with multiple splicing

Background

This gene encodes a member of the RRM family of RNA-binding proteins. The RRM domain is between 80-100 amino acids in length and family members contain one to four copies of the domain. The RRM domain consists of two short stretches of conserved sequence called RNP1 and RNP2, as well as a few highly conserved hydrophobic residues. The protein encoded by this gene has a single, putative RRM domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBPMS Antibody (NBP2-03905)(2)

Publications using NBP2-03905

• Sato K, Shiga Y, Nakagawa Y et al. Ecel1 Knockdown With an AAV2-Mediated CRISPR/Cas9 System Promotes Optic Nerve Damage-Induced RGC Death in the Mouse Retina Invest. Ophthalmol. Vis. Sci. 2018-08-01 [PMID: 30073365] (IHC-Fr, Mouse)
• Sauter MM, Kolb AW, Brandt CR. Toll-like receptors 4, 5, 6 and 7 are constitutively expressed in non-human primate retinal neurons J. Neuroimmunol. 2018-06-11 [PMID: 29954626] (Monkey)

Review for RBPMS Antibody (NBP2-03905) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: macaca mulatta.

reviewed by: Monica Sauter IHC-P macaca mulatta 04/06/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: formalin fixed paraffin embedded macaque retina tissue
• Species: macaca mulatta
• Lot: W0001

Comments

• Comments: heat mediated antigen retrieval (pH 6.0 citrate buffer)
  1:50 dilution of mouse anti-RBPMS (overnight at 4C)
  goat anti-mouse Alexa Fluor 488, 1:400, 1 hr RT

Bioinformatics

• Gene Symbol: RBPMS
• Entrez:
  • 11030(Human)