RBM28 Antibody - BSA Free

Immunohistochemistry-Paraffin: RBM28 Antibody [NBP1-84632] - Analysis in human testis and liver tissues using NBP1-84632 antibody. Corresponding RBM28 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: RBM28 Antibody [NBP1-84632] - Staining of human cerebral cortex shows strong positivity in nucleoli in neurons.

Immunohistochemistry-Paraffin: RBM28 Antibody [NBP1-84632] - Staining of human testis shows strong positivity in nucleoli in cells in seminiferous ducts.

Staining of human testis shows strong positivity in nucleoli in cells in seminiferous ducts.

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Staining of human liver shows very weak positivity in nucleoli in hepatocytes.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RBM28 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RBM28 Antibody - BSA Free (NBP1-84632) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RKLLLSATSGEKGVRIKECRVMRDLKGVHGNMKGQSLGYAFAEFQEHEHALKALRLINNNPEIFGPLKRPIVEFSLEDRRKLKMKELRIQRSLQKMRSKPATGEPQKGQPEPAKDQQQK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM28
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. .
• Control Peptide:

  RBM28 Protein (NBP1-84632PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RBM28 Antibody - BSA Free

• FLJ10377
• RNA binding motif protein 28
• RNA-binding motif protein 28,2810480G15Rik
• RNA-binding protein 28

Background

The protein encoded by the RBM28 gene is a specific nucleolar component of the spliceosomal small nuclear ribonucleoprotein (snRNP)complexes . It specifically associates with U1, U2, U4, U5, and U6 small nuclear RNAs (snRNAs), possibly coordinating their transition through the nucleolus. Mutation in this gene causes alopecia, progressive neurological defects, and endocrinopathy (ANE syndrome), a pleiotropic and clinically heterogeneous disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM28