RBM25 Antibody

Western Blot: RBM25 Antibody [NB100-57506] - Detection of Human RBM25/RED120 on HeLa whole cell lysate usingy NB100-57506. RBM25 was also immunoprecipitated by anaother rabbit anti-RBM25 antibody and NB100-57505.

Immunohistochemistry-Paraffin: RBM25 Antibody [NB100-57506] - Sample: FFPE sections of human testicular seminoma (left) and mouse CT26 colon carcinoma (right). Antibody: Affinity purified rabbit anti- RBM25/RED120 used at a dilution of 1:200 (1 ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RBM25 Antibody Summary

• Description: Novus Biologicals Rabbit RBM25 Antibody (NB100-57506) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human RNA binding motif protein 25 (Arg/Glu/Asp-rich protein, 120 kDa) using the numbering given in entry AAF19255.1 (GeneID 58517).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM25
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBM25 Antibody

• Arg/Glu/Asp-rich protein of 120 kDa
• Arg/Glu/Asp-rich protein, 120 kDa
• fSAP94
• functional spliceosome-associated protein 94
• MGC105088
• MGC117168
• NET52
• Protein S164
• RED120RNA-binding region-containing protein 7
• RNA binding motif protein 25
• RNA-binding motif protein 25
• RNA-binding protein 25
• RNA-binding region (RNP1, RRM) containing 7
• RNPC7
• S164
• Snu71
• U1 small nuclear ribonucleoprotein 1SNRP homolog

Background

RBM25, also identified as hRED120 (for human Arg/Glu/Asp-rich protein of 120 kDa), contains an RNA recognition motif and a PWI nucleic acid binding domain and is suggested to have a role in RNA processing. Alternate designations for RBM25/RED120 include S164, RNPC7, MGC105088, and MGC117168.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM25
• Entrez:
  • 58517(Human)
• Uniprot:
  • AAF19255.1(Human)