RASGRP2 Antibody (3D8) - Azide and BSA Free

Western Blot: RASGRP2 Antibody (3D8) [H00010235-M09] - Analysis of RASGRP2 expression in transfected 293T cell line by RASGRP2 monoclonal antibody (M09), clone 3D8. Lane 1: RASGRP2 transfected lysatE (75.5 KDa). Lane 2: Non-transfected lysate.

ELISA: RASGRP2 Antibody (3D8) [H00010235-M09] - Detection limit for recombinant GST tagged RASGRP2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Ca
• Applications: WB, ELISA
• Clone: 3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RASGRP2 Antibody (3D8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RASGRP2 Antibody (3D8) - Azide and BSA Free (H00010235-M09) is a monoclonal antibody validated for use in WB and ELISA. Anti-RASGRP2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: RASGRP2 (NP_005816, 65 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHPWYIPSSQLAAKLLHIYQQSRKDNSNSLQVKTCHLVRYWISAFPAEFDLNPELAEQIKE
• Specificity: RASGRP2 (3D8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RASGRP2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RASGRP2 Antibody (3D8) - Azide and BSA Free

• Calcium and DAG-regulated guanine nucleotide exchange factor I
• CALDAG-GEFI
• CDC25Lcalcium and diacylglycerol-regulated guanine nucleotide exchange factor I
• Cdc25-like protein
• F25B3.3 kinase-like protein
• guanine exchange factor MCG7
• hCDC25L
• MCG7
• RAS guanyl nucleotide-releasing protein 2
• RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• RAS guanyl-releasing protein 2

Background

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASGRP2 Antibody (H00010235-M09)(1)

Publication using H00010235-M09

• Boudreaux MK, Catalfamo JL, Klok M. Calcium-Diacylglycerol Guanine Nucleotide Exchange Factor I (CalDAG-GEFI) gene mutations associated with loss of function in canine platelets. Transl Res;150(2):81-92. 2007-08-01 [PMID: 17656327]

Bioinformatics

• Gene Symbol: RASGRP2
• Entrez:
  • 10235(Human)
• OMIM:
  • 605577(Human)