RAP80 Antibody - BSA Free

Western Blot: RAP80 Antibody [NBP1-76829] - 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: RAP80 Antibody - BSA Free [NBP1-76829] - Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/mL.

Immunohistochemistry: RAP80 Antibody - BSA Free [NBP1-76829] - Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Po
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RAP80 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RAP80 Antibody - BSA Free (NBP1-76829) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human RAP80. The immunogen is located within amino acids 30 - 80 of RAP80. Amino Acid Squence: DSDGEEPKEENGLQKTK
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UIMC1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  RAP80 Peptide (NBP1-76829PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RAP80 Antibody - BSA Free

• BRCA1-A complex subunit RAP80
• RAP80
• RAP80X2HRIP110
• receptor associated protein 80
• Receptor-associated protein 80
• retinoid x receptor interacting protein
• Retinoid X receptor-interacting protein 110
• RXRIP110
• ubiquitin interaction motif containing 1
• Ubiquitin interaction motif-containing protein 1
• UIMC1
• X2HRIP110

Background

RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UIMC1
• Uniprot:
  • EAW85043(Human)