RAP80 Antibody - BSA Free

Western Blot: RAP80 Antibody [NB100-2346] - Detection of Human RAP80 on HeLa whole cell lysate using NB100-2346. RAP80 was also immunoprecipitated using rabbit anti-RAP80 antibody NB100-2347.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RAP80 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RAP80 Antibody - BSA Free (NB100-2346) is a polyclonal antibody validated for use in WB and IP. Anti-RAP80 Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human Receptor Associated Protein 80 using the numbering given in entry NP_057374.3 (GeneID 51720).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UIMC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAP80 Antibody - BSA Free

• BRCA1-A complex subunit RAP80
• RAP80
• RAP80X2HRIP110
• receptor associated protein 80
• Receptor-associated protein 80
• retinoid x receptor interacting protein
• Retinoid X receptor-interacting protein 110
• RXRIP110
• ubiquitin interaction motif containing 1
• Ubiquitin interaction motif-containing protein 1
• UIMC1
• X2HRIP110

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. TRAP80 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. TRAP80 is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAP80 Antibody (NB100-2346)(8)

Publications using NB100-2346

• Deng J, Zhang T, Liu F et al. CRL4-DCAF8L2 E3 ligase promotes ubiquitination and degradation of BARD1 Biochemical and biophysical research communications [PMID: 35487060]
• Zhu B, Yan K, Li L et al. K63-linked ubiquitination of FANCG is required for its association with the Rap80-BRCA1 complex to modulate homologous recombination repair of DNA interstand crosslinks. Oncogene. 2014-08-18 [PMID: 25132264]
• Hu X, Paul A, Wang B et al. Rap80 protein recruitment to DNA double-strand breaks requires binding to both small ubiquitin-like modifier (SUMO) and ubiquitin conjugates. J Biol Chem 2012-07-01 [PMID: 22689573]
• Hu X, Kim JA, Castillo A et al. NBA1/MERIT40 and BRE interaction is required for the integrity of two distinct deubiquitinating enzyme BRCC36-containing complexes. J Biol Chem 2011-04-01 [PMID: 21282113]
• Morris JR, Boutell C, Keppler M et al. The SUMO modification pathway is involved in the BRCA1 response to genotoxic stress. Nature 2009-12-01 [PMID: 20016594]
• Wang B, Elledge SJ. Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc Natl Acad Sci U S A 2007-12-01 [PMID: 18077395]
• Yan J, Kim YS, Yang XP et al. The ubiquitin-interacting motif containing protein RAP80 interacts with BRCA1 and functions in DNA damage repair response. Cancer Res 2007-07-01 [PMID: 17621610]
• Yan J, Kim YS, Yang XP et al. Ubiquitin-interaction motifs of RAP80 are critical in its regulation of estrogen receptor alpha. Nucleic Acids Res 2007-01-01 [PMID: 17311814]

Bioinformatics

• Gene Symbol: UIMC1
• Entrez:
  • 51720(Human)
• Uniprot:
  • Q96RL1(Human)