RANK/TNFRSF11A Antibody

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Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody induces osteoclast differentiation on mouse splenocytes in the presence of 20 ng/mL recombinant mouse M-CSF (Catalog # 416-ML), in a dose-dependent ...read more
RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartillage primordium, 7 days p.c.) using 10 µg/mL Goat Anti-Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, Func
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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RANK/TNFRSF11A Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse RANK/TNFRSF11A
Gln30-Pro213
Accession # O35305
Specificity
Detects mouse RANK/TNFRSF11A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human RANK is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF11A
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Agonist Activity 0.1-0.4 ug/mL
Publications
Read Publications using
AF692 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RANK/TNFRSF11A Antibody

  • CD265 antigen
  • CD265
  • FEO
  • loss of heterozygosity, 18, chromosomal region 1
  • ODFR
  • ODFROSTS
  • OFE
  • OPTB7
  • Osteoclast differentiation factor receptor
  • PDB2
  • RANK
  • RANKLOH18CR1
  • Receptor activator of NF-KB
  • receptor activator of nuclear factor-kappa B
  • TNFRSF11A
  • TRANCE R
  • TRANCER
  • tumor necrosis factor receptor superfamily member 11A
  • tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Background

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR] and TNFRSF11A) is a member of the tumor necrosis factor receptor family. The full length mouse RANK cDNA encodes a type I transmembrane protein of 625 amino acids (aa) with a predicted 184 aa extracellular domain and a 391 aa cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is widely expressed with the highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF692
Species: Mu
Applications: WB, IHC, Func

Publications for RANK/TNFRSF11A Antibody (AF692)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Flow, Functional Assay, IHC Fluorescence, IHC Not Specified, Neut, WB.


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Flow
(2)
Functional Assay
(1)
IHC Fluorescence
(1)
IHC Not Specified
(1)
Neut
(1)
WB
(1)
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Filter By Species
Mouse
(7)
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Showing Publications 1 - 7 of 7.
Publications using AF692 Applications Species
Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity. Autophagy, 2014;10(11):2036-52. 2014 [PMID: 25483966] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Shimamura M, Nakagami H, Osako M, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice. Proc Natl Acad Sci U S A, 2014;111(22):8191-6. 2014 [PMID: 24847069] (IHC Fluorescence,, Mouse) IHC Fluorescence Mouse
Danzl N, Jeong S, Choi Y, Alexandropoulos K Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6. PLoS ONE, 2014;9(1):e86129. 2014 [PMID: 24465914] (Functional Assay,, Mouse) Functional Assay Mouse
Benasciutti E, Mariani E, Oliva L, Scolari M, Perilli E, Barras E, Milan E, Orfanelli U, Fazzalari N, Campana L, Capobianco A, Otten L, Particelli F, Acha-Orbea H, Baruffaldi F, Faccio R, Sitia R, Reith W, Cenci S MHC class II transactivator is an in vivo regulator of osteoclast differentiation and bone homeostasis co-opted from adaptive immunity. J Bone Miner Res, 2014;29(2):290-303. 2014 [PMID: 24038328] (Flow,, Mouse) Flow Mouse
Baik S, Jenkinson E, Lane P, Anderson G, Jenkinson W Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors. Eur J Immunol, 2013;43(3):589-94. 2013 [PMID: 23299414] (Neut,, Mouse) Neut Mouse
Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse. Dev. Biol., 2009;328(1):127-39. 2009 [PMID: 19298785] (WB,, Mouse) WB Mouse
Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis. J. Immunol., 2007;178(6):3379-89. 2007 [PMID: 17339432] (Flow,, Mouse) Flow Mouse

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Bioinformatics

Gene Symbol TNFRSF11A
Entrez
Uniprot