RanBP2 Antibody

Western Blot: RanBP2 Antibody [NB100-93338] - Detection of Human RanBP2 on HeLa whole cell lysate using NB100-93338. RanBP2 was also immunoprecipitated by rabbit anti-RanBP2 antibody NB100-93337.

• Reactivity: Hu, Pm
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RanBP2 Antibody Summary

• Description: Novus Biologicals Rabbit RanBP2 Antibody (NB100-93338) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2600 and 2650 of human RAN binding protein 2 using the numbering given in entry NP_006258.3 (GeneID 5903).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RANBP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 358 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RanBP2 Antibody

• ANE1
• E3 SUMO-protein ligase RanBP2
• EC 5.2.1.8
• Nuclear pore complex protein Nup358
• nucleoporin 358
• Nucleoporin Nup358
• NUP358TRP2
• p270
• RAN binding protein 2
• Ran-binding protein 2
• RanBP2
• transformation-related protein 2,358 kDa nucleoporin
• TRP1

Background

RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANBP2
• Entrez:
  • 5903(Human)
• Uniprot:
  • P49792(Human)