RALY Antibody - BSA Free

Western Blot: RALY Antibody [NBP2-20065] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: RALY Antibody [NBP2-20065] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-20065] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-20065] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-20065] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RALY Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RALY Antibody - BSA Free (NBP2-20065) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-RALY Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RALY. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Chimpanzee (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RALY
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: IP-Assay dependent
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (85%), Zebrafish (82%), Dog (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RALY Antibody - BSA Free

• Autoantigen p542
• Heterogeneous nuclear ribonucleoprotein C-like 2
• hnRNP associated with lethal yellow protein homolog
• hnRNP core protein C-like 2
• HNRPCL2MGC117312
• P542hnRNP-associated with lethal yellow)
• RNA binding protein (autoantigenic, hnRNP-associated with lethal yellow)
• RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog(mouse))
• RNA-binding protein (autoantigenic)
• RNA-binding protein Raly

Background

In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RALY Antibody (NBP2-20065)(2)

Publications using NBP2-20065

• Xia A, Yue Q, Zhu M et al. The cancer-testis lncRNA LINC01977 promotes HCC progression by interacting with RBM39 to prevent Notch2 ubiquitination Cell death discovery 2023-05-18 [PMID: 37198207] (RIP, Human)
• Xia A, Yuan W, Wang Q et al. The cancer-testis lncRNA lnc-CTHCC promotes hepatocellular carcinogenesis by binding hnRNP K and activating YAP1 transcription Nature Cancer 2022-01-10 [PMID: 35122073] (RIP, Human)

Bioinformatics

• Gene Symbol: RALY
• Uniprot:
  • Q9UKM9(Human)