API5 Antibody (1C2) - Azide and BSA Free

Western Blot: API5 Antibody (1C2) [H00008539-M01] - API5 monoclonal antibody (M01), clone 1C2 Analysis of API5 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Sandwich ELISA: API5 Antibody (1C2) [H00008539-M01] - Detection limit for recombinant GST tagged API5 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

API5 Antibody (1C2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: API5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYERSLQGK
• Specificity: API5 (1C2)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: API5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for API5 Antibody (1C2) - Azide and BSA Free

• AAC-11API5L1
• AAC11fibroblast growth factor 2-interacting factor 2
• Antiapoptosis clone 11 protein
• API-5
• apoptosis inhibitor 5
• Cell migration-inducing gene 8 protein
• Fibroblast growth factor 2-interacting factor
• FIF
• migration-inducing protein MIG8
• Protein XAGL

Background

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for API5 Antibody (H00008539-M01)(1)

Publication using H00008539-M01

• Garcia-Jove Navarro M, Basset C, Arcondeguy T et al. Api5 Contributes to E2F1 Control of the G1/S Cell Cycle Phase Transition. PLoS One. 2013-08-07 [PMID: 23940755]

Bioinformatics

• Gene Symbol: API5
• Entrez:
  • 8539(Human)
• OMIM:
  • 609774(Human)
• Uniprot:
  • AAH17709(Human)