API5 Antibody (1C2)

Images

 
Western Blot: API5 Antibody (1C2) [H00008539-M01] - API5 monoclonal antibody (M01), clone 1C2 Analysis of API5 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Sandwich ELISA: API5 Antibody (1C2) [H00008539-M01] - Detection limit for recombinant GST tagged API5 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Clone
1C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

API5 Antibody (1C2) Summary

Immunogen
API5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYERSLQGK
Specificity
API5 (1C2)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
API5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publication using H00008539-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for API5 Antibody (1C2)

  • AAC-11API5L1
  • AAC11fibroblast growth factor 2-interacting factor 2
  • Antiapoptosis clone 11 protein
  • API-5
  • apoptosis inhibitor 5
  • Cell migration-inducing gene 8 protein
  • Fibroblast growth factor 2-interacting factor
  • FIF
  • migration-inducing protein MIG8
  • Protein XAGL

Background

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for API5 Antibody (H00008539-M01)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol API5
Entrez
OMIM
Uniprot