RALY Antibody

Western Blot: RALY Antibody [NBP1-19111] - Whole cell lysate from HeLa and 293T cells. RALY was successfully immunoprecipitated by rabbit anti-RALY antibody NBP1-19110.

Immunohistochemistry: RALY Antibody [NBP1-19111] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- RALY used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111] - FFPE section of human stomach carcinoma. Affinity purified rabbit anti-RALY used at a dilution of 1:500.

Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111] - FFPE section of mouse renal cell carcinoma. Affinity purified rabbit anti-RALY used at a dilution of 1:100.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RALY Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 256 and 306 of human hnRNP-associated with lethal yellow homolog using the numbering given in entry NP_057951.1 (GeneID 22913).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RALY
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody is useful for western blot. It is not recommended for immunoprecipitation.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RALY Antibody

• Autoantigen p542
• Heterogeneous nuclear ribonucleoprotein C-like 2
• hnRNP associated with lethal yellow protein homolog
• hnRNP core protein C-like 2
• HNRPCL2MGC117312
• P542hnRNP-associated with lethal yellow)
• RNA binding protein (autoantigenic, hnRNP-associated with lethal yellow)
• RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog(mouse))
• RNA-binding protein (autoantigenic)
• RNA-binding protein Raly

Background

In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RALY
• Entrez:
  • 22913(Human)
• Uniprot:
  • NP_057951.1(Human)