Rad51C Antibody

Western Blot: Rad51C Antibody [NB100-1127] - Western blot analysis of Rad51C in HeLa lysate using NB100-1127 at 2 ug/ ml.

Western Blot: Rad51C Antibody [NB100-1127] - (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Rad51C Antibody Summary

• Immunogen: Peptide with sequence RGKTFRFEMQRDL-C corresponding to N-Terminus according to NP_002867.1, NP_478123.1.
• Specificity: This antibody is expected to recognise all reported human isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAD51C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in HeLa lysate (predicted MW of 41 kDa band according to NP_478123).
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rad51C Antibody

• BROVCA3
• DNA repair protein RAD51 homolog 3
• FANCO
• MGC104277
• R51H3
• RAD51 homolog C (S. cerevisiae)
• RAD51 homolog C
• RAD51L2RAD51 (S. cerevisiae) homolog C
• RAD51-like protein 2
• yeast RAD51 homolog 3

Background

The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genome stability. Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad51C Antibody (NB100-1127)(1)

Publication using NB100-1127

• Dosanjh MK, Collins DW, Fan W et al. Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes. Nucleic Acids Res 1998-03-01 [PMID: 9469824]

Bioinformatics

• Gene Symbol: RAD51C
• Entrez:
  • 5889(Human)
• Uniprot:
  • NP_002867(Human)