DNA polymerase alpha Antibody

Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope

Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256] - Analysis of DNA polymerase alpha in mouse A9 fibroblasts that were either Mock infected or were infected with the parvovirus MVM (Minute Virus of Mice). Image courtesy of Dr. Matt Fuller at University of Missouri-Columbia.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DNA polymerase alpha Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 775 and 825 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: DNA polymerase alpha antibody validated for ICC/IF from a verified customer review.
• Theoretical MW: 166 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA polymerase alpha Antibody

• p180
• DNA polymerase alpha 1 catalytic subunit
• DNA polymerase alpha catalytic subunit p180
• DNA polymerase alpha catalytic subunit
• DNA polymerase alpha p180 subunit
• DNA polymerase alpha
• EC 2.7.7.7
• p180
• POLA
• POLA1
• POLADKFZp686K1672
• polymerase (DNA directed), alpha 1
• polymerase (DNA directed), alpha 1, catalytic subunit
• polymerase (DNA directed), alpha
• polymerase (DNA-directed), alpha (70kD)

Background

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and episilon are involved in DNA replication and repair. DNA pol delta and DNA pol episilon are multisubunit enzymes, with DNA pol delta consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for DNA polymerase alpha Antibody (NBP1-47256) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 07/11/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: A9 fibroblasts
• Species: Mouse

Bioinformatics

• Gene Symbol: POLA1
• Entrez:
  • 5422(Human)
  • 18968(Mouse)
• Uniprot:
  • P09884(Human)