DNA polymerase alpha Antibody

Images

 
Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified ...read more
Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256] - Analysis of DNA polymerase alpha in mouse A9 fibroblasts that were either Mock infected or were infected with the parvovirus MVM (Minute Virus of Mice). ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

DNA polymerase alpha Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 775 and 825 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
166 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47256 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA polymerase alpha Antibody

  • DNA polymerase alpha 1 catalytic subunit
  • DNA polymerase alpha catalytic subunit p180
  • DNA polymerase alpha catalytic subunit
  • DNA polymerase alpha p180 subunit
  • EC 2.7.7.7
  • p180
  • POLADKFZp686K1672
  • polymerase (DNA directed), alpha 1
  • polymerase (DNA directed), alpha 1, catalytic subunit
  • polymerase (DNA directed), alpha
  • polymerase (DNA-directed), alpha (70kD)

Background

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and episilon are involved in DNA replication and repair. DNA pol delta and DNA pol episilon are multisubunit enzymes, with DNA pol delta consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for DNA polymerase alpha Antibody (NBP1-47256) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-47256:
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Images Ratings Applications Species Date Details
Immunocytochemistry DNA polymerase alpha NBP1-47256
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reviewed by:
Anonymous
ICC Mouse 07/11/2016
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Summary

ApplicationImmunocytochemistry
Sample TestedA9 fibroblasts
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFollowing MVM infection, NS1 co-localizes with the catalytic subunit of DNA polymerase alpha.Image/data submitted by Matt Fuller at University of Missouri-Columbia.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA polymerase alpha Antibody (NBP1-47256) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
07/11/2016
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol POLA1
Entrez
Uniprot