Rad50 Antibody (684945) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clone: 684945
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Rad50 Antibody (684945) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Rad50
  Leu518-Leu881
  Accession # Q92878
• Specificity: Detects human Rad50 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Rad51 or rhRad53 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rad50 Antibody (684945) [Alexa Fluor® 700]

• DNA repair protein RAD50
• EC 3.6
• EC 3.6.1.15
• EC 3.6.3
• EC 3.6.3.27
• hRad50
• NBSLD
• RAD50 (S. cerevisiae) homolog
• RAD50 homolog (S. cerevisiae)
• Rad50
• RAD502
• RAD50-2

Background

Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes.  It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex.  This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells.  Human Rad50 is 1312 amino acids (aa) in length.  It has an apparent ATP binding site (aa 36-43) plus a coiled-coil region (aa 228-598) followed by a “zinc-hook” domain (aa 635-734) that mediates homodimerization.  There are multiple splice variants.  An alternate start site exists at Met140, there is a single Lys substitution for aa 723-1312, and three Lys substitute for aa 555-1312.  Over aa 518-881, human Rad50 is 96% aa identical to mouse Rad50.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.