Rad50 Antibody (684945) [mFluor Violet 610 SE]

• Reactivity: Hu
• Applications: WB
• Clone: 684945
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

Rad50 Antibody (684945) [mFluor Violet 610 SE] Summary

• Immunogen: E. coli-derived recombinant human Rad50
  Leu518-Leu881
  Accession # Q92878
• Specificity: Detects human Rad50 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Rad51 or rhRad53 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD50
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Rad50 Antibody (684945) [mFluor Violet 610 SE]

• DNA repair protein RAD50
• EC 3.6
• EC 3.6.1.15
• EC 3.6.3
• EC 3.6.3.27
• hRad50
• NBSLD
• RAD50 (S. cerevisiae) homolog
• RAD50 homolog (S. cerevisiae)
• Rad50
• RAD502
• RAD50-2

Background

Rad50 is a 153kDa protein that associates with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, the complex translocates to the nucleus of damaged cells, binds DNA, and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. Overall, Rad50 is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD50