RAD18 Antibody (79B1048.1) - BSA Free

Western Blot: RAD18 Antibody (79B1048.1) [NB100-56523] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate.

• Reactivity: Hu
• Applications: WB
• Clone: 79B1048.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

RAD18 Antibody (79B1048.1) - BSA Free Summary

• Description: Novus Biologicals Mouse RAD18 Antibody (79B1048.1) - BSA Free (NB100-56523) is a monoclonal antibody validated for use in WB. Anti-RAD18 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD18
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4 ug/ml
• Application Notes: In HL60, a 54 kDa band should be detected.
• Control:

  RAD18 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for RAD18 Antibody (79B1048.1) - BSA Free

• EC 6.3.2.-
• hHR18
• hRAD18
• postreplication repair protein hRAD18p
• Postreplication repair protein RAD18
• RAD18 homolog (S. cerevisiae)
• RAD18, S. cerevisiae, homolog
• RING finger protein 73
• RNF73E3 ubiquitin-protein ligase RAD18

Background

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAD18 Antibody (NB100-56523)(2)

Publications using NB100-56523

• Lyakhovich A, Shekhar MP. RAD6B overexpression confers chemoresistance: RAD6 expression during cell cycle and its redistribution to chromatin during DNA damage-induced response. Oncogene. 2004-04-15 [PMID: 14981545] (WB)
  
  Details:
  WB, Figure 6.
• Lyakhovich A, Shekhar MP. Supramolecular complex formation between Rad6 and proteins of the p53 pathway during DNA damage-induced response. Mol Cell Biol. 2003-04-01 [PMID: 12640129] (WB)
  
  Details:
  WB, Figure 2

Bioinformatics

• Gene Symbol: RAD18
• Entrez:
  • 56852(Human)