RAD18 Antibody (79B1048.1) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB
• Clone: 79B1048.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

RAD18 Antibody (79B1048.1) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD18
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for RAD18 Antibody (79B1048.1) [CoraFluor™ 1]

• EC 6.3.2.-
• hHR18
• hRAD18
• postreplication repair protein hRAD18p
• Postreplication repair protein RAD18
• RAD18 homolog (S. cerevisiae)
• RAD18, S. cerevisiae, homolog
• RING finger protein 73
• RNF73E3 ubiquitin-protein ligase RAD18

Background

RAD18 is encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD18