RAB9A Antibody (Mab9)

Images

 
Western Blot: RAB9A Antibody (Mab9) [NB300-621] - Analysis of 25 ug of K562 (lane 1), 293 (lane 2) and Hela (lane 3).
Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Mab9) [NB300-621] - Analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) shows staining in U251 Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and ...read more
Immunohistochemistry-Paraffin: RAB9A Antibody (Mab9) [NB300-621] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues.
Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Mab9) [NB300-621] - Analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) shows staining in Hela Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and ...read more
Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Mab9) [NB300-621] - Analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) shows staining in A2058 Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and ...read more
Immunohistochemistry-Paraffin: RAB9A Antibody (Mab9) [NB300-621] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: RAB9A Antibody (Mab9) [NB300-621] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.

Product Details

Summary
Reactivity Hu, Rt, Bv, Ca, Ha, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IM, IP
Clone
Mab9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

RAB9A Antibody (Mab9) Summary

Immunogen
Recombinant canine rab 9a.
Localization
Cell Membrane (lipid anchor; cytoplasmic side).
Marker
Endosome Marker
Specificity
Recognizes prenylated and non-prenylated rab 9. This does not cross-react with other rab family members.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAB9A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunomicroscopy 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
In WB: Detects an approx. 26 kDa protein representing Rab 9 from K562 cell extract. Electron Microscopy usage was reported in scientific literature (PMID: 21829543). IP usage was reported in scientific literature (PMID: 8389620).
Publications
Read Publication using
NB300-621 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for RAB9A Antibody (Mab9)

  • RAB9A, member RAS oncogene family
  • RAB9RAB9, member RAS oncogene family
  • ras-related protein Rab-9A

Background

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAB9A Antibody (NB300-621)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-621AF405
Alexa Fluor 488 Labeled NB300-621AF488
Alexa Fluor 647 Labeled NB300-621AF647
Alexa Fluor 700 Labeled NB300-621AF700
DyLight 350 Labeled NB300-621UV
DyLight 405 Labeled NB300-621V
DyLight 488 Labeled NB300-621G
DyLight 680 Labeled NB300-621FR
DyLight 755 Labeled NB300-621IR

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Bioinformatics

Gene Symbol RAB9A
Entrez