RAB9A Antibody (Mab9)

Western Blot: RAB9A Antibody (Mab9) [NB300-621] - Analysis of 25 ug of K562 (lane 1), 293 (lane 2) and Hela (lane 3).

Immunocytochemistry/Immunofluorescence: RAB9A Antibody (Mab9) [NB300-621] - Analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) shows staining in U251 Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing RAB9 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: RAB9A Antibody (Mab9) [NB300-621] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues.

Immunocytochemistry/Immunofluorescence: RAB9A Antibody (Mab9) [NB300-621] - Analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) shows staining in Hela Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing RAB9 at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: RAB9A Antibody (Mab9) [NB300-621] - Analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) shows staining in A2058 Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing RAB9 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: RAB9A Antibody (Mab9) [NB300-621] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Immunohistochemistry-Paraffin: RAB9A Antibody (Mab9) [NB300-621] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.

Product Discontinued

RAB9A Antibody (Mab9) Summary

• Immunogen: Recombinant canine rab 9a.
• Localization: Cell Membrane (lipid anchor; cytoplasmic side).
• Marker: Endosome Marker
• Specificity: Recognizes prenylated and non-prenylated rab 9. This does not cross-react with other rab family members.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB9A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunomicroscopy 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000 - 1:5000
• Application Notes: In WB: Detects an approx. 26 kDa protein representing Rab 9 from K562 cell extract. Electron Microscopy usage was reported in scientific literature (PMID: 21829543). IP usage was reported in scientific literature (PMID: 8389620).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for RAB9A Antibody (Mab9)

• RAB9A, member RAS oncogene family
• RAB9RAB9, member RAS oncogene family
• ras-related protein Rab-9A

Background

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAB9A Antibody (NB300-621)(1)

Publication using NB300-621

• Johnson C, Kannan TR, Baseman JB et al. Cellular vacuoles induced by Mycoplasma pneumoniae CARDS toxin originate from Rab9-associated compartments PLoS One 2011-01-01 [PMID: 21829543] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: RAB9A
• Entrez:
  • 9367(Human)
  • 84589(Rat)